【摘要】 目的:研究我国主产地栽培及野生三叶半夏的2种病毒病害发生情况和病毒种类。方法:症状观察法,DAS-ELISA及RT-PCR。结果与结论:侵染我国半夏的病毒主要为黄瓜花叶病毒CMV(cucumber mosaic virus)的天南星科株系和大豆花叶病毒SMV(soybeen mosaic virus)的天南星科株系,后者与以前报道的芋花叶病毒D sMV(dasheen mosaic virus)和SMV都有血清学阳性反应。人工栽培的三叶半夏普遍受到这2种病毒的侵染。其中,在早春检测的浙江宁波栽培半夏21个样品中CMV与SMV的检出率分别为71.4%和14.3%,浙江萧山栽培半夏18个样品中的检出率分别为100%和44.4%;河北栽培半夏21个样品中的检出率分别为61.9%和33.3%;安徽栽培半夏12个样品中的检出率分别为50.0%和41.7%;四川栽培半夏12个样品中2种病毒的检出率均为16.7%;北京栽培半夏16个样品中SMV的检出率为31.3%,未检测到CMV。25个来自全国各地的野生三叶半夏样品中CMV和SMV的平均检出率为20.0%。更多还原

【Abstract】 Objective: To study viruses infecting Pinellia ternata in China.Method: Symptom observation,DAS-ELISA and RT-PCR detection were applied.Result and conclusion: During a survey in early spring,SMV and CMV were both commonly distributed as main viruses infecting P.ternata collected from different areas in China.But DsMV was the virus which infected P.ternate in natural condition.The infection ratio of cultivated P.ternate by SMV and CMV were 71.4% and 14.3% respectively for 21 samples collected from Ningbo,Zhejiang province;100% and 44.4% for 18 samples from Xiaoshan,Zhejiang province;61.9% and 33.3% for 21 samples from Hebei province;50.0% and 41.7% for 12 samples from Anhui province;16.7% and 16.7% for 12 samples from Sichuan province;31.3% and none for 16 samples from Beijing.And the infection ratio of 25 wild samples from different areas of China infected by SMV and CMV were both 20.0%.更多还原