【摘要】 目的:测定人参总皂苷,人参皂苷Rg1,Re,Rb1和人参多糖含量,比较同仁堂红参与高丽红参的质量。方法:建立大孔吸附树脂比色法测定人参总皂苷含量;采用二元梯度洗脱程序的高效液相色谱法测定人参皂苷Rg1,Re,Rb1的含量;优化试验条件,采用苯酚-硫酸显色方法测定人参多糖含量。结果:同仁堂红参中人参总皂苷含量不低于高丽红参中总皂苷的含量;人参皂苷Rg1,Rb1的含量与高丽红参中的含量相近,人参皂苷Re含量略低于高丽红参中的含量,而人参多糖含量则高于高丽红参中的含量。结论:同仁堂红参中人参皂苷含量不低于高丽红参中的含量且人参多糖含量高于高丽红参。建立的测定人参总皂苷、人参皂苷Rg1,Re,Rb1以及人参多糖含量的方法准确、快速、重复性好,可以作为人参质量定量评价的方法,初步比较不同来源的人参品质。更多还原

【Abstract】 Objective: To establish methods for quantitative determination of ginseng saponins,ginsenoside Rg1,Re,Rb1and polysaccarides and compare the qualities of Tongrentang Red Ginseng and Korean Red Ginseng.Method: Macroreticular resin-colorimetric method was developed to determine ginseng saponins and a new HPLC method with gradient eluents was established for determination of ginsenoside Rg1,Re,Rb1.For ginseng polysaccharides,phenol-oil of vitriol colorimetric method was developed and some factors were also optimized.Result: The content of ginseng saponins in Tongrentang Red Ginseng was not lower than that of Korean Red Ginseng.Ginsenoside Rg1 and Rb1 in Tongrentang Red Ginseng were higher than those in Korean Red Ginseng,while Ginsenoside Re was slightly lower than that of Korean Red Ginseng.However,the amount of Ginseng Polysaccharides in Tongrentang Red Ginseng was greater than those in Korean Red Ginseng.Conclusion: The contents of ginseng saponins and ginsenoside Rg1,Re,Rb1in Tongrentang Red Ginseng were not lower than that in Korean Red Ginseng.The methods for determination of ginsenosides and ginseng polysaccharides were quite accurate and reliable to the quality control of Ginseng.更多还原