【摘要】 目的:探讨茅莓提取物(RP)对缺血性脑损伤的神经保护机制。方法:采用大鼠大脑中动脉阻塞造成局灶性脑缺血2 h再灌注24 h模型,TUNEL标记和免疫组化的方法观察茅莓提取物5,10 g.kg-1ig对神经细胞凋亡数目及其相关蛋白Bc l-2,Bax表达的变化。结果:茅莓提取物5,10 g.kg-1显著抑制神经细胞凋亡,增加Bc l-2阳性细胞的表达,降低Bax阳性细胞的表达,Bc l-2/Bax的表达比例增加。结论:茅莓提取物抗凋亡机制可能与增加Bc l-2阳性细胞的表达,降低Bax阳性细胞的表达,提高Bc l-2/Bax有关。更多还原

【Abstract】 Objective: To explore the mechanism of nerve-protecting effects of Rubus parviflolius(RP) on ischemic cerebral injury. Method: The middle cerebral artery was occluded for 2 h to result in the focal ischemic followed by reperfusion of 24 h.TUNEL labeling and immunohistochemical methods were used to investigate changes of neuronal apoptosis and expression of the apoptosis-related proteins after administration of RP.Result: RP decreased the apoptosis of neural cells,the expression of Bax positive cells in the RP groups significantly was decreased as compare with the control group(P<0.05,P<0.01);however,the expression of Bcl-2 positive cells was increased more significantly as compare with the control group(P<0.05,P<0.01);Bcl-2/Bax expression ratio was increased more significantly as compare with the control group(P<0.05,P<0.01).Conclusion: RP has action of resisting neuronal apoptosis after cerebral ischemic-reperfusion.the mechanism of nerve-protecting may be increased Bcl-2 expression,decreased Bax expression and increased Bcl-2/Bax expression ratio.更多还原