【摘要】 目的:建立羌活药材的色谱指纹图谱,为评价羌活药材的质量提供依据。方法:采用Phenom enex Lu-na C18(2)(4.6 mm×250 mm,5μm)色谱柱,乙腈(A)-0.3%醋酸水(B)为流动相梯度洗脱,流速1 mL.m in-1,检测波长330 nm,柱温30℃,洗脱时间70 m in。结果:得到分离度较好的羌活HPLC-UV色谱指纹图谱,标示了7个共有峰。并采用对照品比对和HPLC-MS技术对其部分色谱峰进行了定性鉴别。结论:色谱指纹图谱分析方法为全面控制羌活药材的质量提供了依据。更多还原

【Abstract】 Objective: To establish the chromatographic fingerprints for the identification and quality evaluation of Rhizoma et Radix Notopterygii.Method: Separation was performed on Phenomenex Luna C₁₈（2）（4.6 mm×250 mm,5 μm） column,with mobile phase consisting of acetonitrile（A） and water containing 0.3% acetic acid（B） with gradient elution at the flow rate of 1 mL· min^-1.The UV detection wavelength was 330 nm,column temperature was 30 ℃,and the analysis time was 70 min.Result: A satisfactory fingerprint was obtained by HPLC method,and qualitative analysis of some of the peaks were carried on by HPLC/MS approach.Conclusion: The established method is simple and rapid to identity and differentiate samples of Nhizoma et Radix Notopterygii collected from different sources and might be used as a quality control method for Rhizoma et Radix Notopterygii.更多还原