【摘要】 目的:探讨针刺抑制骨吸收的细胞学机制。方法:40只12月龄雌性SD大鼠,随机分为假手术组、模型组、针刺预防组和针刺治疗组。针刺预防组和针刺治疗组分别在造模术后的3d、3个月开始针刺。取“肾俞”“脾俞”“足三里”“大椎”。自新生SD大鼠四肢长骨分离破骨细胞,接种于24孔培养板中,加入含10%上述各组大鼠血清的培养液培养48h后,抗酒石酸酸性磷酸酶(TRAP)染色并计数TRAP(+)多核细胞数。结果:与假手术组比较,模型组TRAP(+)多核细胞数明显增加(P<0.01);与模型组比较,针刺预防组和针刺治疗组TRAP(+)多核细胞数明显减少(P<0.05)。结论:针刺血清能够减少破骨细胞的数量,预防组和治疗组之间的作用无统计学差异。更多还原

【Abstract】 Objective To observe effects of acupuncture serum of ovariectomized rats on the number of in vitro cultural osteoclast of newborn rats. Methods Forty 12-month-old SD female rats were randomly divided into 4 groups: sham-operation control group, ovariectomized (model) group, acupuncture prevention group and acupuncture treatment group. Osteoclasts isolated from long bone of the newborn SD rat were cultured on 24-well culture plates with DMEM containing 10% the test serum. Forty-eight hours later, the osteoclasts were stained with tartrate resistant acid phosphatase (TRAP) and the number of TRAP-positive multinucleated cells were counted. Results The number of TRAP-positive multinucleated cells in the model group significantly increased as compared with the sham-operation control group (P<0.01), and in the acupuncture prevention group and the acupuncture treatment group significantly declined compared with the model group (P<0.05). Conclusion Acupuncture serum can equally reduce the number of osteoclasts in the two group.更多还原