【摘要】 目的:研究纤维黏连蛋白(FN)整合素途径在调节淋巴瘤细胞对化疗药物敏感性中的作用及姜黄素体外增敏淋巴瘤细胞的作用及其机制。方法:采用四甲基噻唑蓝(MTT)法检测阴性对照组、姜黄素(Cur)组、阿霉素(ADR)组、Cur+ADR组、FN+ADR组、β1整合素抗体(anti-integrin)+ADR组、FN+anti-integrin+ADR组、Cur+FN+ADR组各组细胞的增殖抑制率。金氏公式进行联合用药分析。结果:Cur与ADR合用对Raji细胞呈单纯相加至增强效应。Raji细胞与FN作用后,再给予半数致死剂量的ADR(689.67μmol.L-1),细胞增殖抑制率为32.5%,与未加FN的Raji细胞(抑制率53.1%)相比,两组之间差异极具显著性(P<0.01)。将抗β1整合素抗体阻断Raji细胞上与FN结合的β1整合素,ADR对细胞的抑制率恢复至54.6%。Raji细胞株与FN和6.25μmol.L-1Cur同时培养,ADR对其的抑制率为59.2%,显著高于FN+ADR组(P<0.01)。结论:FN/β1整合素参与调节Raji细胞对ADR的敏感性,Cur可能通过下调整合素的表达抑制FN/β1整合素途径的激活来增加Raji细胞株对化疗药物的敏感性。更多还原

【Abstract】 OBJECTIVE To investigate the effect of FN/β1 integrin pathway on sensitivity of lymphoma cells to chemotherapeutic drugs,and the effect and mechanisms of curcumin on increasing sensitivity of lymphoma cells.METHODS MTT assay was used to study the inhibitory rate of proliferation of lymphoma cells in different groups(control,Cur,ADR,Cur+ADR,FN+ADR,anti-integrin+ADR,FN+anti-integrin+ADR,Cur+FN+ADR).The Jin’s formula was used to analyze the effect of drug combination.RESULTS Simultaneous administration of curcumin combining with adriamycin produced a simple addition or potentiation effect.After cultured with FN,inhibitory rate of lymphoma cells decreased significantly(inhibitory rate 32.5%).After blocking β1 integrin combined with FN by anti-integrin antibody,inhibitory rate recovered.Inhibitory rate of lymphoma cells cultured with curcumin,FN and adriamycin simultaneously was markedly higher than cultured with FN and adriamycin(inhibitory rate 59.2%,P<0.01).CONCLUSION FN/β1 integrin is involved in regulating sensitivity of lymphoma cells to adriamycin.Curcumin can increase the sensitivity of lymphoma cells to chemotherapeautic drugs by inhibiting the action of β1 integrin.更多还原