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烟草无菌苗培养前的种子消毒技术研究

Methods for Seed Sterilization before Aseptic Tobacco Seedling Culture

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【作者】 胡重怡郑少清陈兴江陈丽莉程艳

【Author】 HU Zhongyi,ZHENG Shaoqing,CHEN Xingjiang,CHEN Lili,CHENG Yan(Guizhou Tobacco Research Institute,Guiyang 550003,China)

【机构】 贵州烟草科学研究所贵州烟草科学研究所 贵阳550003贵阳550003

【摘要】 不同消毒处理对烟草种子消毒效果的影响研究表明,在种子消毒之前,经无菌水浸泡24 h(预处理)的比不浸泡的消毒效果好;在10%H2O2、2%有效氯次氯酸钠溶液、1‰升汞3种杀菌剂中,10%H2O2消毒效果最好,种子带菌率为0%,用75%乙醇预处理可降低种子带菌率;3种消毒剂中以10%H2O2处理烟草种子发芽率最高。

【Abstract】 The effects of seed sterilization were studied by different seed sterilization methods.The results showed that sterilization of seeds soaked in aseptic water for 24 hours was better than that without soaking.Under constant temperature,the sterilization effects of three germicides(10%H2O2,2% sodium hypochlorite and 1‰ HgCl2) on tobacco seeds were studied.The results showed that 10%H2O2 had the greatest sterilization effect.The germ-bearing rate of seeds of K326 treated with 10%H2O2 was 0%.Sterilization of seeds soaked in 75% ethanol before seed sterilization decreased germ-bearing rate.The highest germination rate was observed in treatment with 10%H2O2.

【关键词】 烟草无菌苗种子消毒
【Key words】 tobaccoaseptic seedlingseed sterilization
【基金】 贵州不同生态地区烤烟品种优化选择及利用研究(基金编号:2005-2)
  • 【文献出处】 中国烟草科学 ,Chinese Tobacco Science , 编辑部邮箱 ,2007年02期
  • 【分类号】S572
  • 【被引频次】54
  • 【下载频次】1616
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