【摘要】 目的:探讨贫困山区早孕期人巨细胞病毒(HCMV)宫内活动性感染与HCMVUL54基因突变的关系。方法:采用免疫组织化学(SABC)PCR-RFLP的方法检测人绒毛组织中HCMV早期抗原的存在及HCMVUL54基因密码子501是否发生突变。结果:贫困山区早孕期妇女HCMV宫内活动性感染率为18.91%;PCR-RFLP分析结果显示,在早孕期HCMV宫内感染者中UL54501密码子未发生突变。结论:贫困山区早孕期HCMV宫内感染与HCMVUL54基因密码子501突变无关,但不排除其他位点的突变或其他形式异常导致病毒致病性改变存在的可能性。更多还原

【Abstract】 Objective:To explore the relationship between intrauterine human cytomegalovirus(HCMV)infection and UL54 gene mutation during early pregnancy in women of poor mountainous areas.Methods:The early antigen(EA)and UL54 gene mutation in the villus were detected by immunohistochemical method and restriction fragment length polymorphism,respectively.Results:The rate of intrauterine HCMV infection was 18.91%.No UL54 gene mutation was found.Conclusion:There is no relationship between intrauterine HCMV infection and UL54 gene mutation.更多还原