【摘要】 目的:研究在大鼠局灶性脑缺血的动物模型中脑组织白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)的表达变化及血清中IL-6和TNF-α的含量的变化。方法:采用线栓法建立大鼠局灶性脑缺血的动物模型,在缺血后6,12,24,48h断头取脑,并采静脉血。分别以逆转录-聚合酶链式反应和放射免疫方法检测脑组织中IL-6和TNF-αmRNA的变化及血清中IL-6和TNF-α的含量变化。结果:缺血脑组织中IL-6的mRNA的表达水平升高,6h达到高峰,48h恢复到基础水平。血清中IL-6的含量在脑缺血后同样升高,12h达到高峰。而脑组织TNF-αmRNA的表达在12h达到高峰,血清中的TNF-α水平48h内仍继续升高。结论:局灶性脑缺血可以诱发血清和脑组织中IL-6和TNF-α的高表达。更多还原

【Abstract】 Objective:To study the expression of interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) mRNA in ischemic brain tissues and the serum levels of IL-6 and TNF-α in rats with focal brain ischemia. Methods:The rat model of local brain ischemia was established by using intraluminal suture. The rats were killed 6,12,24,and 48 hours after brain ischemia,and the ischemic brain tissue and venous blood were sampled. The expressions of IL-6 and TNF-α mRNA and the serum levels of IL-6 and TNF-α were determined by reverse transcriptional polymerase chain reaction and radioimmunoassay method,respectively. Results:The expressions of IL-6 and TNF-α mRNA in ischemic brain tissue increased,reached the peak after 6 hours,and returned to the baseline after 48 hours. The serum level of IL-6 increased after brain ischemia and reached the peak after 12 hours. The expression of TNF-α mRNA reached the peak after 12 hours. The serum level of TNF-α increased gradually within 48 hours. Conclusion:Focal brain ischemia can induce the high expressions of IL-6 and TNF-α mRNA in ischemic tissue and increase the serum levels of IL-6 and TNF-α.更多还原