【摘要】 目的:改进以高效液相色谱-荧光法测定人血浆中替米沙坦浓度的方法。方法:以萘普生为内标,采用乙酸乙酯提取的方法处理血浆。色谱柱为SymmetryC8,流动相为乙腈-0.01mol.L-1磷酸二氢钾溶液(47∶53),流速为1.0mL.min-1,荧光检测波长为305nm(激发波长)、365nm(发射波长),进样量为20μL。结果:替米沙坦检测浓度在2.5~200ng.mL-1范围内线性关系良好(r=0.9998)。最低检测限为1ng.mL-1。方法的提取回收率为(89.91±10.07)%,日内和日间RSD<10%。样品3次冻融及提取后在24h内稳定性良好。结论:本方法操作简便、灵敏度和精确度高、重现性好,适于替米沙坦的体内药物浓度测定和药动学研究。更多还原

【Abstract】 OBJECTIVE:To improve the HPLC-fluorometric method in the determination of telmisartan in human plasma.METHODS:Using naproxen as internal standard,the plasma was extracted with ethyl acetate.The separation was performed on Symmetry C8 with mobile phase consisted of acetonitrile -0.01mol·L-1 potassium dihydrogen phosphate buffer solution(47∶53) at a flow rate of 1.0ml·min-1.The λEx was set at 305nm and the λEm was set at 365nm,and the sample size was 20μL.RESULTS:The linear range of telmisartan was 2.5～200ng·mL-1(r=0.999 8),with the lowest detectable limit at 1ng·mL-1.The average recovery of extraction was (89.91±10.07)%.Both the intra-day RSD and inter-day RSD were less than 10%.The sample showed a good stability within 24 hours after three freeze-thaw cycles and extractions.CONCLUSI-ON:The method is simple,sensitive,accurate and reproducible,and suitable for the determination and pharmacokinetic study of telmisartan in human plasma.更多还原