【摘要】 目的:采用微射流法制备注射用丝裂霉素C-聚氰基丙烯酸正丁酯纳米粒(MMC-PBCA-NPs)并考察其溶血性。方法:采用乳化聚合法制备MMC-PBCA-NPs粗乳,再经微射流高压均质机制备注射用MMC-PBCA-NPs溶液,采用紫外分光光度法测定其包封率和载药量,观察其粒径及形态等;以家兔进行制剂溶血试验。结果:所制备注射用MMC-PBCA-NPs分散性好,包封率和载药量分别为(85·1±3·8)%和(7·0±0·2)%,平均粒径为(113·5±3·86)nm,形态为圆形,溶血反应为阴性。结论:微射流法用于制备注射用丝裂霉素纳米粒是可行的。更多还原

【Abstract】 OBJECTIVE: To prepare Mitomycin C for injection-Polybutylcyanoacrylate Nanoparticles (MMC-PBCA-NPs) freeze drying preparation by microfluidization and to investigate its hemolytic feature. METHODS: The crude emulsion of MMC-PBCA-NPs was prepared by emulsion polymerization method, from which MMC-PBCA-NPs solution was prepared by microfluidization method; the encapsulation efficiency (EE) and the drug loading (DD) of the preparation were detected by ultraviolet spectrophotometry. The particle size and appearances of which were observed. A hemolytic test was performed on rabbits to observe the blood reaction of the preparation. RESULTS: The prepared MMC-PBCA-NPs freeze drying preparation was uniformly distributed and in round shape, with EE, DD and mean diameter at (85.1±3.8)%, (7.0±0.2)% and (113.5±3.86)nm, respectively. The hemolytic test was negative. CONCLUSION: It is feasible to prepare MMC-PBCA-NPs by microfluidization method.更多还原