【摘要】 目的:建立以高效液相色谱法测定人血浆中阿立哌唑浓度的方法。方法:血浆样品经液-液提取后,采用高效液相色谱法检测。色谱柱为C18,流动相为0．03mol·L-1醋酸铵-乙腈(34:66),流速为0．8mL·min-1,柱温为40℃,检测波长为257nm,灵敏度为0．01 AUFS。结果:阿立哌唑检测浓度线性范围为5．0～600．0ng·mL-1(r=0．999 5),提取回收率均大于90％。结论:该方法灵敏、准确、快速,适用于人血浆中阿立哌唑浓度的检测。更多还原

【Abstract】 OBJECTIVE: To establish a HPLC method for the determination of aripiprazole in human plasma by HPLC. METHODS: The plasma sample was determined by HPLC after subjected to liquid- liquid extraction .The separation was carried out on C18 column with clolumn temperature at 40℃. The mobile phase consisted of 0.03mol·L-1 ammonium acetate-acetonitrile(34:66) with a flow rate of 0.8mL·min-1 and detective wavelength of 257nm.The sensitivity was 0.01 AUFS. RESULTS:The linear range of aripiprazole was 5.0～600.0ng·mL-1(r=0.999 5) .The recovery rate was above 90% .CONCLUSION:The method is sensitive,accurate and rapid,and suitable for the determination of aripiprazole in human plasma.更多还原