【摘要】 为了解我国哺乳动物叠朊基因Prnd的生物信息,本实验采用PCR方法克隆了秦川黄牛、甘肃土种绵羊和汉族人的Prnd,运用分子生物学软件对这些序列进行了分析比较。结果表明,获得的三种哺乳动物的Prnd均位于1个单一的外显子内,与GenBank登录的相应序列具有高度同源性。秦川黄牛与甘肃土种绵羊Prnd的同源性为96.8%,推导氨基酸序列同源性为93.3%。汉族人Prnd与秦川黄牛和甘肃土种绵羊Prnd的同源性均为80%,推导的氨基酸序列同源性均为76.3%。氨基酸一级结构分析显示3种Prnd编码的叠朊均由氨基端的信号肽、中间的成熟蛋白和羧基端的GPI锚结合区组成。二级结构预测表明,3种Prnd编码的叠朊均由3个α-螺旋和2个β-片层组成。本研究获得的这3种主要哺乳动物的Prnd信息为一进步研究叠朊的结构与功能奠定了基础。更多还原

【Abstract】 The mammalian dopple gene Prnds from Qinchuan cattle,sheep native to Gansu province and The Han people were amplified by PCR,sequenced and analyzed with molecular biological softwares.The results showed that the mammalian Prnds existed in a single extron and shared high homologies with the corresponding genes deposited in GenBank.The nucleotide homology between bovine and ovine Prnd was 96.8 % and the similarity in deduced amino acids was 93.3 %.The human Prnd shared 80 % sequence identity with the bovine and ovine Prnd and its deduced amino acids shared 76.3 % homology with that of cattle and sheep.The primary structure analysis showed that dopple protein comprised a N-terminal signal,mature protein and C-terminal region attached by a GPI anchor.The secondary structure prediction showed that dopple protein had three α-helices and two β-strands.This study provided valuable information of mammalian dopple genes in China,and laid foundation for further studies on the gene structure and function.更多还原