【摘要】 本实验以三株芽孢杆菌为材料,采用与沙门氏菌同时接种及先后接种的方法,对其与沙门氏菌的生物拮抗作用进行了研究。结果表明:X1、X2、X3三株芽孢杆菌与沙门氏菌接种后共培养时对沙门氏菌均呈现明显的抑制作用,12h后开始呈现一定的抑制作用,24h时作用最明显,至48h时也还有一定的抑制作用,其抑菌作用依次为:12h时X1>X2>X3,24h时X2>X3>X1,48h时X1>X2,X3对沙门氏杆菌没有抑制作用。接种X1、X2、X3三株芽孢杆菌培养24h后接种沙门氏菌对沙门氏菌均呈现明显的抑制作用,8h后开始呈现一定的抑制作用,12h左右作用最明显,24h后效果却不太明显,其抑菌作用依次为:12h时X1>X3>X2。接种沙门氏菌培养12h后接种X1、X2、X3三株芽孢杆菌对沙门氏菌也呈现明显的抑制作用,6h后开始呈现一定的抑制作用,8h时作用最明显,至24h时也还有一定的抑制作用,其抑菌作用依次为:8h时X2>X1>X3。与对照组比较,统计分析表明:(1)同时接种共培养条件下,接种X1后6h～72h内差异极显著(P<0.01);接种X2后6h～24h和48h～72h内差异极显著(P<0.01),24h～48h差异显著(P<0.05);接种X3后0h～72h内差异不显著(P>0.05)。(2)接种X1、X2、X3培养后接种沙门氏菌条件下,接种X1后0h～72h内差异极显著(P<0.01);接种X2后0h～6h和24h～48h内差异显著(P<0.05),6h～24h为差异极显著(P<0.01);接种X3后0h～72h内差异不显著(P>0.05)。(3)接种沙门氏菌后接种X1、X2和X3条件下,接种X1后6h～8h和12h～36h内差异极显著(P<0.01),8h～12h为差异显著(P<0.05);接种X2后6h～8h和12h～36h内差异极显著(P<0.01);接种X3后24h～36h内差异显著(P<0.05)。更多还原

【Abstract】 The effects of 3 Bacillus strains, X1, X2 and X3 inhibiting the growth of enteric Salmonella were investigated by the co-incubation, pre-incubation and post-incubation method. The result revealed that the 3 Bacillus strains showed different ability to inhibit the growth of Salmonella after co-incubated with X1, X2 and X3 respectively. Powerful inhibiting ability to Salmonella occurred at 12 h-24 h in the means of co-incubation both of simultaneously and successively, and at 8 h-12 h in the means of incubation Salmonella first and then co-incubation X1, X2 and X3. Statistical data showed that by co-incubation, pre-incubation and post-incubation X1 with Enteric Salmonella, the inhibiting effects were most obviously difference(P<0.01)at 0 h-72 h, 0 h-72 h, and both of 6 h-8 h and 12 h-36 h, respectively; and were obviously difference (P<0.05) at 8 h-12 h by post-incubation X1 with enteric Salmonella. The inhibiting effects were most obviously difference (P<0.01) at both of 0 h-24 h and 48 h-72 h, 6 h-24 h, and both of 6 h-8 h and 12 h-36 h. By co-incubation, pre-incubation and post-incubation X2 with enteric Salmonella; and were obviously difference (P<0.05) at 24 h-48 h, both of 0 h-6 h and 24 h-48 h by co-incubation and pre-incubation X2 with enteric Salmonella. By co-incubation, pre-incubation and post-incubation X3 with enteric Salmonella, the inhibiting effects were obviously difference (P<0.05) at 24 h-36 h just in post-incubation group only, no obviously differences were observed in the other two groups.更多还原