【摘要】 目的探讨双歧杆菌拮抗辐射损伤的时效、量效和理想的拮抗分子。方法实验动物均选用SPF级C57BL小鼠,用不同药物和不同照射剂量处理后,建立急性放射损伤模型。WBC计数:用不同数量级浓度的双歧杆菌菌悬液对小鼠灌肠28 d后,5.0 Gy照射后3 d、6 d和10 d采取小鼠外周血检测WBC数。30 d小鼠生存率:用1×109CFU/m l双歧杆菌菌悬液灌肠28 d后,用一次性8.0 Gy60Coγ射线全身照射,建立急性放射损伤模型,每日上下午各观察小鼠1次,记录死亡动物数和死亡日期。抗氧化指标检测:用双歧杆菌菌体3种不同成分LTA、WPG和EPS,连续7 d腹腔注射小鼠后6.0 Gy照射,检测肝组织CAT和SOD的活性。结果WBC计数结果显示:双歧杆菌提高了辐射损伤WBC的最低值(P<0.05),随着剂量增加有上升趋势,理想的双歧杆菌菌悬液浓度为1×109CFU/m l。30 d小鼠生存率试验结果显示:双歧杆菌将小鼠生存率提高了43.3%。抗氧化结果显示:双歧杆菌菌体3种成分均具有抗氧化作用,尤其LTA抗氧化作用最为明显(P<0.05)。结论双歧杆菌对辐射损伤具有拮抗作用,其较为理想的双歧杆菌菌悬液浓度数量级为109CFU/m l,双歧杆菌细胞壁的3种成分中抗辐射较为理想的效应分子是LTA。更多还原

【Abstract】 Objective To study the antagonistic effect of bifidobacterium on radiation damage,it includes time-effect relationship,dose-effect relationsiip and the best effector.Methods To establish acute radiation damage pattern,C57BL mice in SPF grade were divided into different groups by different ways and irradiation doses.The test of white blood cell count was finished by the different number of bifidobacterium suspension coloclysis and general irradiation with 5.0 Gy γ ray to establish acute radiation damage pattern.The 30 survival rate was tested and reported after the bifidobacterium suspension coloclysis of 1×109CFU/ml and 5.0 Gy ray to establish acute radiation damage pattern number in every day.The antioxygen indicatrix of SOD and CAT were detected by the successive intraperitoneal injection with different thallus component for 7 days.Results The number of white blood cell count was significantly increased in bifidobacterium group compared to radiation damage group.The ideal suspension density was 1×109CFU /ml.The 30 survival rate increased 43.3% in bifidobacterium group compared to radiation damage group.The activity of SOD and CAT significantly increased in LTA group compared to others.Conclusions 1×109 clonal formation unit per milliliter is the ideal suspension concentration to bifidobacterium on radiation damage. And lipoteichoic acid is the ideal bifidobacterium effector molecule on radiation damage.So bifidobacteria possess the ability of antagonistic effect on radiation damage.更多还原