【摘要】 目的:研究粉防己碱对运动性蛋白尿发生时肾脏细胞凋亡的干预作用。方法:将大量购进的符合实验要求的雄性Wistar大鼠先按每组15只随机分为安静对照组和安静干预组,其余大鼠每天行负重游泳训练连续2h,待复制出确切的运动性蛋白尿模型后随机纳入实验,并按每组15只将其分为过度训练对照组和过度训练干预组。安静对照组和过度训练对照组每日用pH7.4的磷酸盐缓冲液1.5ml/kg灌胃,安静干预组和过度训练干预组每日灌注粉防己碱30mg/kg,连续用药10d。安静对照组和安静干预组给药期间安静自由喂养,而过度训练对照组和过度训练干预组则需在给药后4h按原方案行负重游泳训练2h。用末端标记法检测各组大鼠肾组织细胞凋亡,采用图像分析仪计算肾脏细胞凋亡率。结果:过度训练对照组尿蛋白持续增高,肾皮质可见大量凋亡细胞,凋亡百分率明显高于其他各组(P<0.01)。过度训练干预组未见尿蛋白持续增高现象,肾组织仅见少量凋亡细胞数,凋亡百分率虽高于安静干预组与安静对照组,但仍明显低于过度训练对照组(P<0.01)。结论:过度训练可导致尿蛋白持续增加和肾脏细胞凋亡,粉防己碱可阻止过量运动所致的肾脏细胞凋亡,进而减轻运动性蛋白尿,对运动性肾损伤有保护作用。更多还原

【Abstract】 The purpose of this study was to analyze the intervention of tetrandrine on the apoptosis of renal cell with exercise albuminria.Mass purchasing male Wistar rats according with the requirement of this experiment was randomly divided into sedentary control group and sedentary interventional group with 15 rats per group,the remainder did loaded-swimming every day for 2 hours,until replicating the model of exercise albuminria.The rats with exercise albuminria were randomly divided into overtraining control group and overtraining interventional group with 15 rats per group.Sedentary control group and overtraining control group with pH7.4 PBS 1.5ml/kg per day,perfusion,sedentary interventional group and overtraining interventional group with tetrandrine 30mg/kg per day,perfusion,with the medicine for 10 days.The sedentary control group and sedentary interventional group peaceful feeding,while the overtraining control group and overtraining interventional group doing loaded-swimming every day fore 2 hours after with the medicine 4 hours.Detecting the in situ cell apoptosis by TUNEL and calculating the ratio of renal cell apoptosis by image dissector.The result shows that in overtraining control group,the urinary protein increased significantly in concomitant,the mass cell of apoptosis have already been clearly observed on the Proximal tubule,the ratio of renal cell apoptosis increased significantly that in other group(P<0.01).The overtraining interventional group without the phenomena of urinary protein increased,the ratio of renal cell apoptosis higher than sedentary control group and sedentary interventional group,those effects still remains have very significantly difference compared with the overtraining control group(P<0.01).Conclusions It concludes that overtraining can lead to increase the urinary protein in concomitant and evoke apoptosis of renal cell.Tetrandrine is able to protect the renal tubular epithelial cell apoptosis induced by overtraining.The above results can indicate that tetrandrine has antagonism when renal injury induced by violent movement.更多还原