【摘要】 为探讨香菇β-葡聚糖(lentinan,LTN)对雏鸡免疫增强作用的机理,从体内和体外2种途径研究了LTN对雏鸡脾和胸腺淋巴细胞以及腹腔巨噬细胞NO产生的影响。体内试验的4组21日龄雏鸡分别腹腔注射1 g/L的LTN、10 g/L的LTN、20 g/L的硫乙醇酸钠和生理盐水各2 mL,3 d后测脾和胸腺淋巴细胞以及腹腔巨噬细胞产生NO的量;分别以50、100、200、400 mg/L的LTN刺激经硫乙醇酸钠活化的巨噬细胞,测定其NO的生成量。结果显示,1 g/L和10 g/L的LTN均能刺激体内脾和胸腺淋巴细胞以及腹腔巨噬细胞产生NO;不同浓度的LTN均能使体外巨噬细胞NO的生成量增加。证实,适度促进免疫细胞NO的生成可能是LTN的主要免疫作用机理之一。更多还原

【Abstract】 To evaluate the mechanism of immune enhancement of β-lentinan(LTN) in chickens,both in vitro and in vivo trials were carried out to study the effect of LTN on NO productive capacity of the splenic and thymus lymphocytes and abdominal macrophage.One-hundred-twenty 21day-old chickens were randomly divided into 4 groups which were injected abdominally with 2 mL of 1 g/L LTN,10 g/L LTN,20 g/L sodium thioglycolate and normal saline solution,respectively.The NO production of the splenic and thymus lymphocytes and abdominal macrophage were measured on day 3 post-injection.And the macrophage activated by sodium thioglycolate were stimulated with LTN at level of 50 mg/L,100 mg/L,200 mg/L and 400 mg/L respectively and the NO production by macrophages were measured.In consequence,1 g/L and 10 g/L LTN stimulated the splenic and thymus lymphocytes and macrophages to produce NO.Different concentrations of LTN were able to stimulate macrophages to increase the production of NO.Moderate stimulation of immunocytes to produce NO may be one of the most important immunoactive mechanisms by LTN in chickens.更多还原