【摘要】 采用RT-PCR技术对8株禽呼肠孤病毒(ARV)σNS基因进行了扩增,将获得的PCR产物分别与pMD18-T载体连接。测序结果表明,所构建的克隆质粒中均含有相应的σNS蛋白基因,大小为1 107bp。经DNAStar软件分析,除R1株外,其他ARV毒株的σNS基因核苷酸及其推导氨基酸序列与标准毒株S1133和1733株的同源性很高。Antheprot 5.0蛋白分析软件的分析结果表明,σNS蛋白无跨膜区,拥有较多的疏水区和抗原位点,可作为诊断候选蛋白。更多还原

【Abstract】 The σNS gene of 8 avian reovirus(ARV) strains was amplified by RT-PCR.The PCR pro-duct was inserted into pMD18-T vector and sequenced.Sequence analysis showed that the recombinant plasmid carried σNS gene of 1 107 bp in size.The analysis by DNAStar software showed that σNS gene shared very high identity with the published sequences of S1133 and 1733 in GenBank except R1 isolate.The analysis using Antheprot 5.0 protein software showed that the deduced amino acid sequence from the σNS gene had good hydrophobicity and antigenicity and no transmembrane region,and the σNS protein can be used as candidate antigen for serological diagnosis.更多还原