【摘要】 目的:探讨双电极绑定条件下记录大鼠在体海马CA1区长时程增强的可行性。方法:雄性Wistar大鼠乌拉坦麻醉;脑立体定位仪上埋置脑室导管;安装自制的刺激/记录绑定电极;引导基础性场兴奋性突触后电位(fEP-SP);强直刺激诱导长时程增强(LTP)。结果:绑定后的刺激和记录电极能可靠地引起海马CA1区fEPSP,fEPSP的出现率几乎100%;基础性fEPSP记录可保持长时间稳定;高频刺激成功诱导出LTP并维持达3h以上,诱导率约67%;双脉冲易化记录稳定、可靠;脑室注射β淀粉样蛋白(Aβ)对LTP显示出明显的压抑作用。结论:采用双电极绑定技术进行在体海马LTP记录简便易行、节省资源、引导fEPSP和诱导LTP的成功率较高,有望成为一项重要的研究学习和记忆机制的电生理辅助手段。更多还原

【Abstract】 Aim:To study the feasibility of long-term potentiation(LTP)recording in the CA1 area of the rat in vivo with electrodes-bin-ding technique.Methods:Anesthetizing Wistar rats with urethane and fixing the animal on the stereotaxic device for acute surgery;implanting cannula into lateral cerebral ventricle;inserting self-made bound stimulating/recording electrodes into hippocampal CA1 area;recording basal field excitatory postsynaptic potential(fEPSP)and tetanus-induced long term potentiation(LTP).Results:fEPSPs were reliably induced by using the stimulating/recording electrodes-binding technique,and the appearance rate of fEPSP was nearly 100%;basal fEPSP recording was very stable,lasting for long time enough to finish all experiment;high frequency stimulation(HFS)successfully induced LTP,which maintained more than three hours,the inductivity is about 67%;paired-pulse facilitation(PPF)recording was also stable;intracerebroventricular(i c v)injection of amyloid beta suppressed HFS-induced LTP evidently.Conclusion:The electrodes-binding technique for recording hippocampal LTP in vivo is quite simple and convenient.The experimental resource can be saved,and the rates of fEPSP appearance and LTP induction are kept high.Therefore,it is promising for this technique to be one electrophysiological auxiliary method in the research of learning and memory.更多还原