【摘要】 在获得了105份转豌豆铁蛋白基因(Fer)水稻转化体的基础上,通过连续7代自交纯化,并结合GUS标记辅助选择,得到了82份独立转基因纯系。经稻米矿质元素检测,发掘到2份富铁转基因种质(Fer34和Fer65),其精米全铁含量为野生型亲本秀水11的4.82倍和3.46倍。进一步鉴定表明,所筛选到的2份富铁转基因种质的外源基因(Fer)遗传简单,增加精米粉铁含量明显,对植株重要农艺性状、其他矿质营养、精米外观和食用品质等均无明显的负作用,是较为难得的富铁转基因优良新种质。此外,还就该2份富铁种质的实用价值及其应用前景进行了讨论。更多还原

【Abstract】 A total of 105 transgenic rice lines independently transformed with a pea ferritin gene(Fer) were previously obtained.After seven generations of self-crossing and GUS marker assisted selection,82 homozygous transgenic rice lines were used to evaluate their potential applications as high-iron rice germplasm.Two high-iron transgenic rice lines Fer34 and Fer65,with the iron content in the milled rice 4.82 and 3.46 times of the wild parent Xiushui 11 were identified.Further analysis indicated that the exogenous Fer gene was highly expressed,and inherited as a single locus.The transgene had no negative effect on the agronomic traits of rice plants,other mineral nutritional components,appearance and eating quality of the milled rice,indicating that they were elite high-iron breeding lines.Furthermore,the practical application and further studies facilitating utilization of the two elite breeding lines were discussed.更多还原