节点文献
芜菁花叶病毒CP基因反向重复植物表达载体的构建及遗传转化
Construction and Transformation of Inverted Repeat of Turnip Mosaic Virus Coat Protein Gene
【摘要】 针对芜菁花叶病毒外壳蛋白基因构建反向重复表达载体,利用农杆菌介导法将其导入四九菜心,PCR检测共获得13个阳性转化株,遗传转化效率为2%。
【Abstract】 The construct using inverted repeat sequences of the coat protein gene of TuMV was designed,and introduced into Sijiucaixin via Agrobacterium tumefaciens-mediated transformation system.13 positive transformants were obtained through PCR analysis.The transgenic efficiency was up to 2 %.
【基金】 国家自然科学基金项目(30471189)
- 【文献出处】 中国蔬菜 ,China Vegetables , 编辑部邮箱 ,2007年02期
- 【分类号】S634.5
- 【被引频次】3
- 【下载频次】275