【摘要】 目的:探讨丁香酚吸入引起昆明鼠学习记忆的行为学改变及其机制。方法:建立小鼠嗅球摘除模型,分组对小鼠进行水迷宫实验、嗅觉辨识记忆实验及免疫组化,观察各组小鼠海马、皮质等脑区的尼氏染色,以及ChAT、GABA、Glu等神经递质变化。结果:丁香酚吸入组的小鼠水迷宫实验、嗅觉辨识记忆实验结果明显优于其他组,免疫组化实验结果示丁香酚吸入小鼠的皮质、海马的ChAT、Glu等神经递质有明显增加,而GABA无明显改变。结论:丁香酚能通过嗅觉通路改善小鼠的学习记忆,其机制可能与嗅觉通路中多种神经递质的改变有关。更多还原

【Abstract】 Objective: To study mechanisms of Eugenol improving learning and memory through olfactory pathway on Kunming(KM) mice. Method: We established olfactory bulbectomy models, divided different groups. The mice were detected with mouse water maze test and olfaction discrimination memory test. Then, each mouse was sacrificed and the brain was removed. The slices of hippocampus and cortex were stained with Nissl′s staining and detected the expression of ChAT, GABA and Glu by immunohistochemistry. Result: Eugenol inhaling group can strengthen mouse water maze test, olfaction discrimination memory test compared to the other groups, and ChAT, Glu in hippocampus and cortex had significantly increased, but GABA had not. Conclusion: The effects of eugenol′s improvement on learning and memory may act through olfactory pathway. The mechanisms is related to the changing of the brain′s neurotransmitters.更多还原