【摘要】 采用0.1,1.0,10.0μg/mL的微囊藻毒素-RR(MC-RR)处理烟草BY-2悬浮细胞,测定了细胞活力、细胞内蛋白质含量、可溶性糖含量、硝态氮含量及总磷含量,并且检测了酸性磷酸酶(ACP)的活力变化情况.结果表明,中、高浓度毒素处理细胞2d后,细胞活力及蛋白质含量与对照相比均显著下降.高浓度MC-RR处理降低了胞内可溶性糖的含量,暴露2d后仅为对照的45.57%;低浓度MC-RR处理在后期增加了胞内可溶性糖含量.高浓度毒素处理细胞4d后,细胞内硝态氮含量显著低于对照;中、低浓度毒素处理细胞7d后降低了胞内硝态氮含量.3组毒素处理均降低了胞内总磷含量,到实验结束时,低、中、高浓度处理组的胞内磷含量分别为对照的74.98%、76.47%和84.00%.3组处理组ACP活力与对照相比呈现先降低后升高的趋势.更多还原

【Abstract】 Adopting 0.1, 1.0, 10.0μg/mL microcystin-RR (MC-RR) to treat tobacco BY-2 suspension cells, the cell viability, contents of protein, soluble sugar, nitrate-nitrogen, and total phosphorus in cells were determineded; and the viability change condition of acid phosphatase (ACP) was detected. The cell viability and protein content after the cells were treated by middle and high concentration toxin for 2d decreased markedly compared with the contrast. The soluble sugar content decreased by treating of high concentration MC-RR, after exposure of 2d, it was only 45.57% of the contrast. The soluble sugar content increased in the later period of low concentration MC-RR treating. After the cells were treated by high concentration toxin for 4d, the content of nitrate-nitrogen in cells were lower markedly than the contrast, after the cells were treated by middle and low concentration toxin for 7d, the content of nitrate-nitrogen in cells were lowered. 3 groups of toxin treating all lowered the total phosphorus content. At the end of test, the phosphorus contently of low, middle, high concentration treating groups were 74.98%, 76.47% and 84.00% of the contrast respectively. The ACP activity of 3 treating groups compared with the contrast appeared the tendency of first lowering then raising.更多还原