【摘要】 目的探讨p53基因突变与人乳头状肉瘤病毒16(HPV16)感染在食管癌发病中的作用及相互关系。方法应用病例-病例研究方法进行分析,采用聚合酶链反应(PCR)、单链构像多态性(SSCP)、测序等分子生物学技术对110例食管癌组织标本中p53基因的突变与HPV16感染进行了检测。结果110例食管癌组织标本p53基因突变率为49.1%,其中外显exon5-6,exon7,exon8-9的突变率分别为19.1%,27.3%,17.2%。食管癌组织HPV16的检出率为49.1%。吸烟患者p53基因突变率(61.4%)明显高于非吸烟患者p53基因突变率(48.2%),差异有统计学意义;淋巴结转移患者p53基因突变率(65.2%)明显高于无淋巴结转移患者p53基因突变率(37.5%);HPV16阳性者中,p53突变率是40.7%,HPV16阴性者仅为57.1%,两者差异无统计学意义。结论HPV16感染可能是食管癌高发区的危险因素;p53基因突变与吸烟、淋巴结转移有明显相关性;HPV16感染和p53基因突变可能是2个独立的事件。更多还原

【Abstract】 Objective To investigate the relationship between p53 mutations and human papillomavirus 16(HPV16)infections in esophageal cancer.Methods p53 mutations and infections were examined by polymerase chain reaction(PCR)and single strand conformation polymorphism(SSCP)and DNA direct sequencing analysis in the 110 exophageal cancers.The case-case study was used for factor anlysis.Results The rate of mutations in exon5-6,exon7,exon8-9 were 19.1%,27.3% and 17.2% respectively.The rate of p53 gene mutation was 61.4% in smoke groups and 48.2% in nosmoker groups.There was a significant difference between them.The rate of p53 gene mutation was 65.2% in lymph node metastasis groups and 37.5% in nolymph node metastasis groups.There was significant difference between them.The rate of p53 gene mutation was 40.7% in HPV16 negative groups and 57.1% in HPV16 positive groups,There was no association between HPV16 status and p53 mutations.Conclusion High-risk HPV plays an important role in the development of esophageal cancer.There is a significant difference between p53 mutations and lymph node metastasis.This result may predict that the cases could be deteriorated after the operation.p53 gene mutations may play an important role in the mechanism of esophageal cancer and support the relationship between p53 mutations and HPV infection not to be mutually exclusive.更多还原