【摘要】 目的从氧化应激角度探讨硫酸镍(NiSO4)对大鼠睾丸细胞线粒体及微粒体的毒性作用。方法健康性成熟Wistar雄性大鼠32只,随机分为4组:生理盐水(NS)组,硫酸镍(NiSO4)1.25,2.50,5.00 mg/kg组,等容积腹腔注射染毒,1次/d,连续30 d。制备睾丸组织匀浆,离心提取线粒体和微粒体,采用分光光度法检测睾丸细胞线粒体及微粒体中脂质过氧化物(LPO)、总抗氧化能力(T-AOC)、活性氧(ROS)水平的变化。结果染毒组与对照组比较,大鼠睾丸脏器系数无明显变化(P>0.05)。镍可引起睾丸组织中LPO、ROS含量升高,与对照组比较差异有统计学意义(P<0.05);各染毒组T-TOC均低于对照组(P<0.05)。结论镍对大鼠睾丸细胞的损伤可能与其所致的睾丸细胞线粒体及微粒体氧化应激效应增强有关。更多还原

【Abstract】 Objective To explore the mitochondria and microsome of testis cells caused by nickel sulfate in rate.Methods Thirty-two male Wistar rats with sexual maturation were divided into four groups randomly,and three groups of rats were administrated intraperitoneally with nickel sulfate daily at doses 1.25,2.50,5.00 mg/kg respectively for 30 days,but the control group was administrated with saline in same volume according to the rats’weight.The mitochondria and microsome of testis cells were prepared by centrifugation technique to detect the levels of lipid peroxidation(LPO),total antioxide capacity(T-AOC),reactive oxygen species(ROS) in spectrophotometry.Results The organ coefficient of testis exposed to NiSO4 had no change compared wth the control group(P<0.05).The LPO and ROS contents in testis of exposure groups were higher than that of control group(P>0.05),but the T-AOC levels of the exposure groups were decreased compared with the control group(P<0.01).Conclusion The rat’s testis damage is related to the enhancement of oxidative stress of mitochondria and microsome caused by nickel sulfate.更多还原