节点文献

硫酸镍致大鼠睾丸细胞线粒体及微粒体损伤

Oxidative damage of mitochondria and micriosome in testis cells induced by nickel sulfate in rats

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 孙应彪陈建华刘一亚宋媛朝朱玉真

【Author】 SUN Ying-biao,CHEN Jian-hua,LIU Yi-ya,et al.School of Public Health,Lanzhou University(Lanzhou 730000,China)

【机构】 兰州大学公共卫生学院卫生毒理学研究所兰州水泵总厂职工医院兰州大学公共卫生学院卫生毒理学研究所 兰州730000兰州730000

【摘要】 目的从氧化应激角度探讨硫酸镍(NiSO4)对大鼠睾丸细胞线粒体及微粒体的毒性作用。方法健康性成熟Wistar雄性大鼠32只,随机分为4组:生理盐水(NS)组,硫酸镍(NiSO4)1.25,2.50,5.00 mg/kg组,等容积腹腔注射染毒,1次/d,连续30 d。制备睾丸组织匀浆,离心提取线粒体和微粒体,采用分光光度法检测睾丸细胞线粒体及微粒体中脂质过氧化物(LPO)、总抗氧化能力(T-AOC)、活性氧(ROS)水平的变化。结果染毒组与对照组比较,大鼠睾丸脏器系数无明显变化(P>0.05)。镍可引起睾丸组织中LPO、ROS含量升高,与对照组比较差异有统计学意义(P<0.05);各染毒组T-TOC均低于对照组(P<0.05)。结论镍对大鼠睾丸细胞的损伤可能与其所致的睾丸细胞线粒体及微粒体氧化应激效应增强有关。

【Abstract】 Objective To explore the mitochondria and microsome of testis cells caused by nickel sulfate in rate.Methods Thirty-two male Wistar rats with sexual maturation were divided into four groups randomly,and three groups of rats were administrated intraperitoneally with nickel sulfate daily at doses 1.25,2.50,5.00 mg/kg respectively for 30 days,but the control group was administrated with saline in same volume according to the rats’weight.The mitochondria and microsome of testis cells were prepared by centrifugation technique to detect the levels of lipid peroxidation(LPO),total antioxide capacity(T-AOC),reactive oxygen species(ROS) in spectrophotometry.Results The organ coefficient of testis exposed to NiSO4 had no change compared wth the control group(P<0.05).The LPO and ROS contents in testis of exposure groups were higher than that of control group(P>0.05),but the T-AOC levels of the exposure groups were decreased compared with the control group(P<0.01).Conclusion The rat’s testis damage is related to the enhancement of oxidative stress of mitochondria and microsome caused by nickel sulfate.

【关键词】 硫酸镍睾丸线粒体微粒体氧化应激
【Key words】 nickel sulfatetestismitochondriamicrocomeoxidative stress
  • 【文献出处】 中国公共卫生 ,Chinese Journal of Public Health , 编辑部邮箱 ,2007年04期
  • 【分类号】X174
  • 【被引频次】16
  • 【下载频次】179
节点文献中: 

本文链接的文献网络图示:

本文的引文网络