【摘要】 目的研究八氯二丙醚对体外培养中国仓鼠肺细胞(CHL)DNA合成影响。方法采用DNA荧光定量测定方法,观察不同染毒浓度(0.250,0.187,0.125,0.062,0.046,0.031μg/ml)对CHL细胞DNA合成影响。结果八氯二丙醚浓度为0.062,0.046μg/ml时DNA合成量与对照组比差异有统计学意义,表现为合成增加(P<0.05),浓度为0.250,0.187,0.125μg/ml时,则表现为合成抑制(P<0.05),且呈剂量-反应关系(P<0.05);0.250μg/ml浓度在脱离染毒后2,4,6,12 h内DNA合成量持续下降。结论在低浓度时(0.062,0.046μg/ml),八氯二丙醚可引起CHL细胞DNA合成能力增加,而在高浓度时(0.250,0.187,0.125μg/ml)则表现为DNA合成抑制,且这种抑制由DNA损伤引起。更多还原

【Abstract】 Objective To study effects of octachlorodipropyl ether on DNA synthesis in CHL in vitro.Methods Fluorimetric quantification assay was applied to detect DNA synthesis of CHL cells treated with different concentrations(0.250,0.187,0.125,0.062,0.046,0.031 μg/ml).Results In the concentration gruop of 0.062 and 0.046 μg/ml,DNA synthesis quantities were significantly different from the control group(P<0.05),However,DNA synthesis inhibition and good dose-response relationships were shown in the gruop of 0.250,0.187 and 0.125 μg/ml.DNA synthesis quantities of 0.250 μg/ml group indicated continual descent trend when it was treated without octachlorodipropyl ether in 2～12 horus.Conclusion Low concentration of octachlorodipropyl ether could lead to the increase of DNA synthesis ability in CHL cells.Concentrations of 0.250,0.187,and 0.125 μg/ml may lead to DNA synthesis inhibition,which was caused by DNA damage.更多还原