【摘要】 目的研究人类白细胞抗原(HLA)-DR3、DR7、DR13、DR53等位基因与乙型肝炎病毒(Hepatitis Bvirus,HBV)宫内感染的关系,探讨HBV宫内感染的遗传易感性,确定HBV宫内感染的易感基因和保护基因。方法采用聚合酶链反应/序列特异性引物(PCR-SSP)技术检测HBsAg阳性孕妇及其新生儿各187例外周静脉血血块中HLA-DR3、DR7、DRl3、DR53等位基因型分布及频率。结果(1)宫内感染组孕妇HLA-DR3基因频率为24.14%(7/29),显著高于非宫内感染组孕妇的6.33%(10/158)(P=0.007)。其余各表型比较,差异无统计学意义(P>0.05)。(2)宫内感染组新生儿HLA-DR3基因频率为17.24%(5/29),显著高于非宫内感染组新生儿的5.06%(8/158)(P=0.033)。其余各表型比较,差异无统计学意义(P>0.05)。(3)母婴HLA-DR3同阳性在宫内感染组与宫内未感染组间比较差异有统计学意义(P=0.049);母婴HLA-DR7、DR13、DR53同阳性在宫内感染组与宫内未感染组间比较,差异无统计学意义(P>0.05)。结论HBsAg阳性孕妇及其胎儿同时或任一携带HLA-DR3,易导致HBV宫内感染;HLA-DR3是HBV宫内感染的易感基因。更多还原

【Abstract】 Objective To study the association between HLA-DR3,DR7,DR13,DR53 and HBV intrauterine infection to probe into the susceptibility to intrauterine infection of hepatitis B virus and to find its predisposing genes and protecting genes.Methods HLA-DR3,DR7,DR13,DR53 were detected by the technique of polymerase chain reaction-sequence specific primers(PCR-SSP) in maternal and neonatal blood clot.Results(1) The frequency of HLA-DR was 24.14% in women with intrauterine infection,which was higher than that(6.33%) in women without intrauterine infection(P=(0.007)).There was no association between HLA-DR7,DR13,DR53 positive in pregnant women and HBV intrauterine infection(P>0.05).(2) The frequeney of HLA-R of newborns was 17.24% in women with intrauterine infection,which is higher that(5.06%) in women withotll infection(P=0.033).There was no association between HLA-DR7,DR13,DR53 positive in newborns and HBV intrauterine infection(P>0.05).(3)There was significant association between HLA-DR3 concordance and HBVintrauterine infection(P=0.049).There was no association between DR7,DR13,DR53 concordance and HBV intrauterine infection(P>0.05).Conclusion HLA-DR3 carriers of pregnant women and their newborns have susceptibility to HBV intrauterine infection.HLA-DR3 is the predisposing gene of HBV intrauterine infection.更多还原