【摘要】 目的:构建人卵透明带蛋白(huZP3)基因原核表达载体。方法:利用PCR法扩增出含人卵透明带蛋白编码基因,先将其克隆到pGEM-T载体,进行序列测定和分析,然后将huZP3蛋白编码基因亚克隆至原核表达载体pGEX4T-1上,酶切鉴定。结果:获得一个核苷酸长度约为1200bp的基因,同源比较结果表明,与GenBank(NCBI:M60504)公布的ZP3基因序列有100%的同源性。酶切表明ZP3基因正确插入原核表达载体pGEX4T-1。结论:成功构建出含ZP3基因的原核表达载体。更多还原

【Abstract】 Objective:To construct expression vector which can express human zona pellucida in prokaryotic cells.Methods:The ZP3 gene was amplified by PCR. The amplified DNA fragment was cloned into pGEM-T vector for sequencing analysis. The ZP3 gene was then subcloned into prokaryotic expression vector pGEX4T-1.Results:A gene of 1 200 bp was obtained and showed a 100% homology with ZP3 gene sequence published in GenBank (NCBI: M60504). ZP3 gene was inserted into prokaryotic expression vector pGEX4T-1 correctly.Conclusion:ZP3 protion expression vector is constructed successfully.更多还原