【摘要】 目的:探讨我院2005年儿科院内感染常见致病菌的分布及对抗生素的耐药情况,为临床合理用药提供依据。方法:3001例院内感染患儿,根据感染部位不同,采集血液、痰、分泌物、便及尿液作为检测标本,采用VITEK-2全自动细菌鉴定仪进行细菌鉴定;药物敏感试验使用K-B法;采用WHONET软件对耐药数据进行分析。结果:共检测标本3024份,分离出细菌232株,革兰阴性菌为首要致病菌,占60%,其中肺炎克雷伯菌、大肠埃希菌、液化沙雷菌列前3位。革兰阳性菌占40%,其中凝固酶阴性耐甲氧西林葡萄球菌的检出率为35%。大肠埃希菌产超广谱β-内酰胺酶(ESBLs)为55%,肺炎克雷伯菌产ESBLs为51%。革兰阳性球菌敏感药物为万古霉素(耐药率0)、替考拉宁(耐药率0),对红霉素、克磷霉素、青霉素、苯唑西林的耐药率均大于50%;革兰阴性杆菌较敏感药物为亚胺培南、美罗培南(耐药率0),对头孢二代、三代的耐药率已高达50%。结论:儿科院内感染常见致病菌耐药较严重,合理运用抗生素对控制儿童院内感染及指导临床用药十分重要。更多还原

【Abstract】 Objective:To study the situation of pathogenic bacteria of pediatric infection in hospital,so can guide clinical doctors use antibiotic rationally.Methods:The urine、secretions、stools、blood and sputum of children patients were collected to be used as the specimen.Identification of bacteria performed by VITEK-2;Antimicrobial susceptibility of the clinical isolates collected were tested by Kirby-Baue rmethod.The results were analyzed according to NCCLS by WHONET 5 software.Results:Among 232 strains bacteria from 3024 samples,gram negative bacteria was 60%,E-coli,Klebsiella,serratia liquefaciens were ranked respectively.Gram positive bacteria was 40%,in which MRCNS was 35%.ESBLs-positive in E coli was55 %,in klebsiella pneumoniae was 51%..Drugs of sensitivity for Gram positive coccus were vancomycin(0),teicoplanin(0);Resistant rates of erythromycin,clindamycin,pneicilin were more than 50%,and the drugs of sensitivity for Gram negative were imipenem(0%),merropenem(0);Resistant rates of the second and third cephalosporins were high to 50%.Conclusion:Bacterial resistance is a serious problem.It is instructive that use antibiotic rationally for controlling children infection in hospital.更多还原