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利用3种抗体流式分选母血中胎儿细胞的比较

Comparison of isolating fetal erythroblasts from maternal blood by three monoclonal antibodies using flow cytometry

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【作者】 徐晓燕陈汉平相文佩张铭蒋荣珍沈红玲郭玉珍

【Author】 XU Xiao-Yan, CHEN Han-Ping, XIANG Wen-Pei, et al.Department of Gynecology and Obstetrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, China

【机构】 华中科技大学同济医学院附属同济医院妇产科华中科技大学同济医学院附属同济医院妇产科 (湖北武汉)430030(湖北武汉)430030

【摘要】 目的比较单克隆抗转铁蛋白受体抗体(CD71McAb)、单克隆抗血型糖蛋白A抗体(GPAMcAb)和抗血红蛋白-ξ抗体(ξ-Hb)3种胎儿有核红细胞(FRBCs)特异性抗体在流式细胞仪上分选母血中胎儿细胞的检出率,评价分选胎儿细胞的最适方法。方法收集55例孕妇外周血,利用淋巴细胞分离液从中分离出单个核细胞层后,每例标本分为5组,分别加入不同抗体第1组CD71Mchb单用;第2组GPAMcAb单用;第3组ξ-Hb单用;第4组CD71+GPA联用;第5组CD71+GPA+ξ-Hb联用,孵育后流式细胞仪进行分选,应用PEP-PCR检测分选后的胎儿细胞的SRY基因。结果5组FNRBC的阳性细胞检出率及鉴定符合率分别为2.74%,77.14%;5.25%,65.4%;0.22%,95.7%;0.72%,98.6%;0.02%,60%。结论利用CD71McAb和GPAMcAb双重标记胎儿细胞进行分选或者利用抗ξ-Hb特异性McAb分选FRBCs是更有效的分选方法。

【Abstract】 Objective:To compare the gain rates of isolating fetal erythroblasts from maternal blood by three monoclonal antibodies using flow cytometry, and to estimate the best method of isolating fetal cells.Methods:55 peripheral blood samples were collected from pregnant women.Each sample was divided into 5 groups. Different monoclonal antibodies were added respectively, and then they were analyzed on flow cytometry after incubated. PEP-PCR was used to detect the SRY gene of the isolated fetal cells.Results:The gain rates and the concordance rates of the five groups were: 2.74%, 77.14%; 5.25%, 65.4%; 0.22%, 95.7%; 0.72%, 98.6%;0.02%,60%.Conclusion:The methods of isolating fetal erythroblasts using anti-ξ-Hb McAb alone or anti-CD71 McAb and anti-GPA MoAb jointly are more effective.

【基金】 湖北省自然科学基金资助项目(2001ADBl30、2002AA301C86);湖北省计划生育委员会科研项目资助项目(2002.6);湖北省科委资助项目(2004AA301C92)
  • 【文献出处】 中国妇幼保健 ,Maternal and Child Health Care of China , 编辑部邮箱 ,2007年04期
  • 【分类号】R714.5
  • 【被引频次】6
  • 【下载频次】178
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