【摘要】 目的研究Aβ42寡聚体对PC12细胞的损伤作用,观察Aβ42寡聚体的聚集状态的变化。方法用不同浓度Aβ42寡聚体作用于PC12细胞,并与Aβ42作用的PC12细胞、正常PC12细胞进行对照研究。应用MTT、LDH检测细胞活性,原位末端标记(TUNEL)方法检测细胞凋亡。应用原子力显微镜(AFM)观察Aβ42寡聚体的聚集状态、PC12细胞表面形貌变化。结果MTT、LDH在Aβ42寡聚体组的改变较对照组、Aβ42组差异有显著意义(P<0.05);TUNEL法测定Aβ42寡聚体组凋亡率高,较对照组、Aβ42组差异有显著意义(P<0.05)。Aβ42寡聚体对PC12细胞的损伤作用随浓度增高而增加。原子力显微镜可观察到Aβ42寡聚体、Aβ42的不同聚集状态。结论Aβ42寡聚体的细胞损伤作用与浓度相关,且强于Aβ42。利用原子力显微镜可观察Aβ的聚集变化。更多还原

【Abstract】 Objective To explore the role of cytotocity of Aβ oligomer to PC12 cell.Methods The PC12 cells were treated with Aβ42 and Aβ42 oligomer.To detect cytoactive by MTT and LDH,and neuronal apoptosis by TdT-mediated dUTP-biotin nick end labeling(TUNEL).Effects of AFM on pathogenesis investigation of Aβ and Aβ oligomer study were outlined.Results The evidence for neuron cytotocity was found by MTT and LDH,but some early signs of neuronal apoptosis were found by TUNEL method.Significant increase in apoptosis had been shown in Aβ42 oligomer group in analysis of TUNEL as compared with Aβ42 group(P<0.05).Aβ42 fibril and oligomer were observed and determined by AFM.Conclusions The cells damage effects of Aβ42 oligomer were stronger than Aβ42,and correlated with concentrations.AFM has provided useful insights at all stages of Aβ fibrillization and make therapeutic strategy possible.更多还原