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AgNO3在铁皮石斛组织培养中抗衰老作用

Effects of AgNO3 on caducity-resistant from tissue culture of Dedrobium officinale

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【作者】 李进进廖俊杰许继勇麦瑜玲

【Author】 LI Jin-jin1, LIAO Jun-jie1, XU Ji-yong2, MAI Yu-ling2(1. Department of Food and Bio-technolgy, Guangdong Industry Technical College, Guangzhou 510300, China; 2. Shantou Zhongshu Flower Co., Ltd., Shantou 515041, China)

【机构】 广东轻工职业技术学院食品与生物工程系汕头市中蔬花卉有限公司汕头市中蔬花卉有限公司 广东广州510300广东广州510300广东汕头515041

【摘要】 目的解决铁皮石斛在组织培养过程中的衰老问题。方法在原球茎增殖培养基、丛芽增殖培养基和生根培养基中分别加入AgNO30、1、2mg/L,测定乙烯的产生量,观察试管苗生长情况。结果AgNO3抑制乙烯的产生,提高了原球茎的增殖速度和丛芽的分化效率,明显促进种苗的生长,提高移栽成活率2倍,表现出抗衰老功能。结论1mg/LAgNO3可以作为铁皮石斛组织培养的抗衰老剂。

【Abstract】 Objective To solve the caducity question of Dendrobium officinale during the tissue-culture process. Methods Added the different concentration of 0.1 and 2 mg/L AgNO3 into the PLB proliferation medium, buds proliferation medium, and rooting medium separately. The quantity of the ethylene production was to be determined and the situation of the tube seedling growth was to be observed. ResultsAgNO3 can suppress the production of the ethylene, enhance the multiplication speed of PLB and split-up efficiency the clump of bud, and obviously promote the seedling′s growth. It showed that the survival ratio of transplant could enhance as many as two times and display the merit of caducity-resistant. ConclusionAgNO3(1 mg/L) can be taken as the caducity-resistant reagent of D. officinale during the tissue-culture processing.

【基金】 广东省2005年科技资助项目[粤科计字(2005)193号];汕头市2005年科技资助项目[汕市财文(2005)219号]
  • 【文献出处】 中草药 ,Chinese Traditional and Herbal Drugs , 编辑部邮箱 ,2007年06期
  • 【分类号】R282.1
  • 【被引频次】18
  • 【下载频次】544
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