【摘要】 目的研究金龙胆草Conyza blinii的化学成分及其抗溃疡活性。方法采用溶剂提取、重结晶和硅胶、聚酰胺柱色谱方法分离纯化,根据氢谱、碳谱、质谱以及与对照品共薄层色谱鉴定化合物的结构。用幽门结扎大鼠模型进行抗溃疡活性筛选。结果从金龙胆草的乙醇提取物中分离鉴定了8个化合物:5,8,3′,4′-四羟基-7-甲氧基黄酮(Ⅰ)、5,8,4′-三羟基-7,3′-二甲氧基黄酮(Ⅱ)、β-谷甾醇(Ⅲ)、菠甾醇(Ⅳ)、正二十六烷酸(Ⅴ)、5,7-二羟基-3,3′,4′-三甲氧基黄酮(Ⅵ)、5,7-二羟基-3,8,4′-三甲氧基黄酮(Ⅶ)和苦蒿素(Ⅷ)。苦蒿素能明显减少模型大鼠胃溃疡面积,明显减少胃黏膜组织中的MDA的水平。结论化合物Ⅰ~Ⅲ和Ⅴ~Ⅶ为首次从该植物中分离得到。苦蒿素对幽门结扎大鼠胃溃疡的形成有明显抑制作用,其可能作用机制与抑制胃黏膜组织中MDA的生成有关。更多还原

【Abstract】 Objective To study the chemical constituents in the whole herb of Conyza blinii and seek its antiulcerogenic principles. MethodsCompounds were separated and purified by solvent extraction, re-crystallization, and chromatography on silica gel and polyamide column. Their structures were elucidated on the basis of 1H-NMR, 13C-NMR, and MS spectral data and co-TLC with authentic samples. Pylorus-ligated rat model was used for the evaluation of antiulcerogenic activity. ResultsEight compounds were identified: 5, 8, 3′, 4′-tetrahydroxy-7-methoxy flavone (Ⅰ), 5, 8, 4′-trihydroxy-7, 3′-dimethoxy flavone (Ⅱ), β-sitosterol (Ⅲ), spinasterol (Ⅳ)、n-hexacosanic acid (Ⅴ), 5, 7-dihydroxy-3, 3′, 4′-trimethoxy flavone (Ⅵ), 5, 7-dihydroxy-3, 8, 4′-trimethoxy flavone (Ⅶ), blinin (Ⅷ). Blinin could significantly narrow the ulcer area and lower the level of gastric mucous MDA. ConclusionCompounds Ⅰ-Ⅲ and Ⅴ-Ⅶ are obtained from the title plant for the first time. Blinin is found to possess significant antiulcer property.更多还原