【摘要】 目的:观察姜黄素对高脂血症兔主动脉表达NF-κB、VCAM-1、VEGF影响,探讨姜黄素抗动脉粥样硬化的可能机制。方法:♂新西兰家兔30只,喂养高脂饮食8周后,随机分为5组:对照组、姜黄素小剂量(10mg/kg/d)、中剂量(50mg/d)、大剂量(100mg/kg/d)和氟伐他汀组。第12周麻醉后处死动物主动脉标本,用免疫组化法检测NF-κB、VCAM-1、VEGF蛋白的表达。结果:1)姜黄素可以降低高脂饮食兔血清TC,TG,LDL水平。2)姜黄素可以抑制NF-κB、VCAM-1、VEGF的表达,作用强度与剂量大小相关(P<0.05)。结论:姜黄素具有抗动脉粥样硬化作用,可能与抑制NF-κB活性,减少VCAM-1、VEGF表达,从而抑制炎症和新生血管有关。更多还原

【Abstract】 AIM:To observe the effect of curcumine on expression of NF-κB,VCAM-1 and VEGF in aorta of hyperlipemia rabbits and approach its probably anti-atherosclerosis mechanisms.METHODS:30 healthy New Zealand rabbits were fed with high fat diet for 8 weeks and randomly divided into five group:control group,curcumine low dose group(10 mg/kg/d),middle dose group(50 mg/kg/d),large dose group(100 mg/kg/d)and fluvastatin group.All animals were anaesthetized and killed in the 12th weeks to obtain aorta tissue and measured expression of NF-κB,VCAM-1,VEGF through immunohistochemistry.RESULTS:1)Curcumine could low serum level of TC,TG,LDL of rabbits with high fat diet.2)Curcumine could inhibit expression of NF-κB,VCAM-1,VEGF,which role was positive correlation with dose(P<0.05).CONCLUSION:Curcumine has some effect on atherosclerosis which may be relevant to inhibiting activation of NF-κB,decreased expressions of VCAM-1、VEGF and suppressive inflammation action and neoformative vessel.更多还原