【摘要】 目的:研究微胶囊化儿茶素对阿霉素肾病大鼠血管内皮生长因子(VEGF)表达的影响。方法:将120只雌性SD大鼠随机分为对照组、肾病组、地塞米松组、维生素E组、儿茶素组和微胶囊组共6组,尾静脉1次性注射阿霉素(5 mg/kg BW)制备肾病模型;利用酶联免疫吸附试验测定血清与尿中VEGF含量;利用免疫组织化学方法检测肾组织中VEGF表达。结果:实验第4周末(维生素E组除外)与第6周末,各组大鼠尿、血清及肾组织中VEGF含量均显著高于对照大鼠、显著低于肾病大鼠(均P<0.01),实验末,微胶囊组大鼠尿与血清中VEGF含量明显低于儿茶素组(分别P<0.01,P<0.05),微囊组大鼠肾组织VEGF表达低于儿茶素组,但无显著差异。微囊化儿茶素治疗组24 h尿蛋白排泄量显著低于儿茶素治疗组(P<0.05),24 h尿蛋白排泄与尿、血清及肾组织中VEGF含量均显著正相关(P<0.01)。结论:儿茶素降低阿霉素肾病大鼠尿蛋白排泄可能是通过降低VEGF的排泄与分泌实现的,儿茶素微囊化后,有助于提高其降低VEGF的排泄与分泌,减少尿蛋白的排泄。更多还原

【Abstract】 AIM: To study the effect of microcapsulated catechin on vascular endothelial grower factor(VEGF) expression in rats with adriamycin induced-nephrotic syndrome.METHODS: 120 female SD rats were randomly distributed in control group,nephrotic group,dexamethason group,vitamin E group,catechin group and microcapsule group.Rat with nephrotic syndrome were induced by injection of adriblastine(5 mg/kg BW).VEGF concentrations in serum and urine were detected by ELISA assay.VEGF expression in kidney was measured by immunohistochemistry assay.RESULTS: At the end of 4th week and 6th week,VEGF concentration in other groups in kidney,serum and urine were higher than that in control(all P<0.01),and were lower than that in nephritic group(exclude Vit E group,all P<0.01).Serum and urine VEGF concentrations in microcapsule group were lower than those in catechin group(P<0.01,P<0.05,respectively).VEGF expression in microcapsule group in kidney was lower than that in catechin group,but it was not significant different.Urinary protein excretion at 24 h in microcapsule group was lower than that in catechin group(P<0.05),there was positive correlation among urine protein,serum VEGF level,urine VEGF concentration and renal VEGF expression at 24 h(all P<0.01).CONCLUSION: Catechin decreases urinary protein excretion in the rats with nephrotic syndrome through reducing the expression and secretion of VEGF.Microcapusulated catechin is benefit in decreasing the expression and secretion of VEGF.更多还原