【摘要】 目的:探讨炎症刺激因子TNF-α介导炎症时上调血管内皮细胞Tie2基因的机制。方法:采用RT-PCR、定量PCR以及Western blotting方法检测TNF-α作用于原代人主动脉内皮细胞株(HAECs)、原代人肺动脉内皮细胞株(HPAECs)时Tie2基因及ETS转录因子mRNA及蛋白的表达。结果:TNF-α可增加血管内皮细胞Tie2基因的表达。TNF-α不能增加内皮细胞HAECs和HPAECs的NERF-2、ELF表达;TNF-α刺激后约24h第1次检测到ESE-1 mRNA表达。延长刺激作用时间后,发现在18-36h之间可测到ESE-1 mRNA表达,24h为高峰,而蛋白质表达在18h极弱,36h达峰值。检测中所观察到ESE-1表达在Tie2表达之前且表达模式类似。结论:Tie2基因在TNF-α刺激时的上调与NERF-2或ELF介导无关,ESE-1可能在炎症因子刺激后Tie2的转录调节中起作用。更多还原

【Abstract】 AIM: To explore the mechanism of tyrosine kinase receptors with immunoglobulin and EGF homology domains receptors 2(Tie 2) gene up-regulation in vascular endothelial cell induced by TNF-α.METHODS: By applying with RT-PCR, quantitative PCR and Western blotting, mRNA and protein expressions of Tie 2 and some ETS transcription factors were detected in primary human aortic endothelial cells (HAECs) and primary human pulmonary artery endothelial cells (HPAECs). RESULTS: TNF-α increased the expression of Tie 2 in vascular endothelium cells. TNF-α did not increase the expression of New ETS related factor-2(NERF-2) and E74-like factor-1(ELF1) in HAECs and HPAECs. ESE-1 mRNA began to increase at 24 h. After extending stimulation time, it was found that ESE-1 mRNA was detected at 18-36 h and the peak was at 24 h, while protein expression was very weak at 18 h, and the peak was at 36 h. The expression of ESE-1 occurred before Tie 2 expression and the mode was similar.CONCLUSION: Tie 2 gene is not related to up-regulation of NERF-2 or ELF when stimulated with TNF-α. ESE-1 may play a role in transcription regulation of Tie 2 after inflammation.更多还原