【摘要】 目的:评估褪黑素预处理对油酸引起的大鼠急性肺损伤的拮抗作用。方法:24只大鼠随机分为生理盐水组、油酸组和褪黑素预处理组3组,检测大鼠肺泡灌洗蛋白、肺的湿干比重、丙二醛、超氧化物岐化酶水平,并进行肺的病理组织学检查。结果:(1)静脉注射0.15 mL/kg油酸引起肺泡灌洗蛋白、肺的湿干比重显著增高(P<0.01),病理组织学检查发现有肺出血、肺水肿、肺泡间隔增厚以及肺泡腔出现炎性细胞,导致严重的急性肺损伤;(2)提前60 min腹腔注射20 mg/kg褪黑素明显减轻上述的症状,与丙二醛的降低(P<0.01)和超氧化物岐化酶的升高(P<0.01)相一致。结论:褪黑素预处理可通过清除和防止自由基的形成,进一步降低肺泡毛细血管膜通透性的增加,从而减轻油酸引起的大鼠急性肺损伤。提示褪黑素可能在急性肺损伤的防治中是有效的。更多还原

【Abstract】 AIM: To assess the protective role of melatonin(MEL) in a rat model of oleic-induced acute lung injury.METHODS: Twenty-four rats were randomly allocated to three groups as follows: saline(NS) injection group,oleic acid(OA) injection group and MEL plus OA injection group,the lavage protein,lung wet-to-dry weight ratio,malondialdehyde(MDA) content,superoxide dismutase(SOD) activity and lung histopathology were examined.RESULTS:(1) Injection 0.15 mL/kg of OA led to a severe acute lung injury(ALI),characterized by significantly increasing in lavage protein,lung coefficient(P<0.01),and by histopathological alterations which presented hemorrhage,edema,thickened alveolar septum and the existence of inflammatory cells in alveolar spaces;(2) Infusion of MEL(20 mg/kg,intraperitoneally for 60 min before the oleic acid) markedly alleviated above-mentioned symptom induced by OA,consistent with decrease of MDA level(P<0.01) and the increase of SOD activty(P<0.01).CONCLUSION: Pre-treatment with MEL can attenuate the OA-induced ALI in rats via cleaning and preventing the formation of free radicals and further lessening the increase of alveolocapillary membrane permeability,these data suggest that MEL may be effective in the prevention of ALI.更多还原