【摘要】 目的:观察LPS攻击小鼠IL-1β、IL-6、IL-4、IL-10的动态变化规律及八肽胆囊收缩素(CCK-8)对其表达的影响。方法:将小鼠分为4组:对照组、LPS组(腹腔注射LPS)、LPS+CCK-8组(注射LPS前30 min腹腔注射CCK-8)及CCK-8组(单独注射CCK-8)。用ELISA及PT-PCR方法检测各组小鼠血清、肺组织中IL-1β、IL-6、IL-4、IL-10的含量及mRNA的表达情况。结果:LPS攻击可使小鼠血清及肺组织中IL-1β、IL-6、IL-4、IL-10蛋白及mRNA的表达增加,预先注入CCK-8可显著抑制IL-1β、IL-6的表达,并使IL-4、IL-10的表达进一步增加。结论:CCK-8可能通过抑制LPS攻击小鼠IL-1β、IL-6的表达和进一步增加IL-4、IL-10的表达参与抗炎反应过程,从而减轻LPS引起的肺组织炎症反应。更多还原

【Abstract】 AIM:To observe the effects of cholecystokinin octapeptide(CCK-8) on the expression of proinflammatory cytokines IL-1β,IL-6 and anti-inflammatory cytokines IL-10,IL-4 in LPS-attacked mice.METHODS: Kunming mice were randomly assigned and injected intraperitoneally with LPS alone or/and CCK-8 at different time points.The expression of IL-1β,IL-6,IL-10 and IL-4 in the serum and lung tissues were assayed by ELISA and RT-PCR.RESULTS: The expression of IL-1β,IL-6,IL-10 and IL-4 were upregulated in LPS-attacked mice.Pre-treatment of CCK-8 decreased both IL-1β and IL-6 expression and augmented IL-10 and IL-4 expression in LPS-attacked mice.CONCLUSIONS: CCK-8 exerts an anti-inflammatory effect by inhibiting the expression of IL-1β,IL-6 and increasing the expression of IL-10,IL-4 in LPS-attacked mice,which could alleviate the inflammatory response in lung tissue.更多还原