【摘要】 目的探讨内皮细胞蛋白C受体(EPCR)与系统性红斑狼疮(SLE)并发狼疮性肾炎(LN)的关系。方法采用酶联免疫吸附试验(ELISA)和反向聚合酶链反应(RT-PCR)技术分别对SLE患者和并发LN的SLE患者血浆中可溶性EPCR(sEPCR)水平和外周血循环系统内皮细胞蛋白C受体mRNA的表达进行检测。结果SLE患者的sEPCR平均水平(261±13ng/ml)比正常对照组的sEPCR平均水平(167±11ng/ml)明显增高(P<0.001);并发LN的SLE患者sEPCR水平(312±21ng/ml)比LN的SLE患者的sEPCR平均水平(261±13ng/ml)明显增高(P<0.001)。SLE患者组(132.5±32.6mg/L)及并发LN的SLE患者组(201±33.5mg/L)的EPCRmRNA表达均明显高于正常对照组(88.6±29.5mg/L)的EPCRmRNA表达(P<0.005),且SLE患者组与并LN的SLE患者组间EPCRmRNA表达有显著性差异(P<0.005)。结论外周血循环系统EPCR升高可能是一个早期发现LN的标志物,EPCR在狼疮性肾炎发病中可能发挥重要作用。更多还原

【Abstract】 Objective To investigate the relation between the endothelium protein C receptor and lupus nephritis(LN). Methods The soluble endothelial protein C receptor (sEPCR) in plasma and EPCR mRNA in peripheral blood circulating endothelial protein C receptor(EPCR) were detected by enzyme linked Immunosorbent assay(ELISA) and reverse transcription-polymerase chain reaction(RT-PCR) in patients with systemic lupus erythematosus(SLE),LN and normal control,respectively.Results The mean levels of sEPCR was significantly higher in SLE patients(261±13 ng/ml) than that in controls(167±11 ng/ml)(P<0.001) The mean levels of sEPCR was significantly higher in LN patients(312±21 ng/ml)than SLE patients(261±13 ng/ml)(P<0.001).The expression of EPCR mRNA was significantly higher in SLE patients[(132.5±32.6)mg/L] and LN patients(201±33.5 mg/L)than that in normal controls(88.6±29.5 mg/L),and it existed obvious differences between in SLE patients and LN patients(P<0.005). Conclusion The increase of EPCR might be a marker that could be used to detect early stage LN and EPCR played important roles in pathogenesis of LN.更多还原