【摘要】 利用农杆菌介导的转化方法将菜豆铁结合蛋白基因(PvFer)导入’粉红908’番茄基因组中。经PCR、PCR-Southern、Southern blotting检测证实,外源基因已整合到2株番茄基因组中。半定量式RT-PCR检测表明,外源菜豆铁结合蛋白基因在2株转基因植株中得到了表达。测定番茄果实和叶片中铁、锌、锰含量表明,转ferritin基因株系T1的叶片铁含量提高了174%,果实中的铁含量提高了37%,锌在果实中提高了119%,而锰在果实中的含量却降低了56%。更多还原

【Abstract】 In this study,the bean iron-binding protein gene(PvFer)was introduced into the genome of tomato Fenhong 908 by Agrobacterium-mediated genetic transformation.The results of PCR,PCR-Southern and Southern blotting analysis confirmed that foreign ferritin gene was integrated into the genome of two tomato plants.RT-PCR revealed that the foreign ferritin gene was expressed in the two tomato plants.Measurement of Fe,Zn,Mn contents in fruits and leaf of the transgenic tomato plants suggested that iron content in leaves of T1 transgenic line was approximately 2.7 times that of the non-transformed leaves,and iron content in fruits was increased by 37%;zinc content in fruits was increased by 119%,but manganese content in fruits of T1 transgenic line was decreased by 56%.更多还原