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四川主栽茶品种亲缘关系的RAPD分析
RAPD Analysis on the Genetic Relationships of Tea Cultivars Grown in Sichuan
【摘要】 利用RAPD标记对四川主要产茶区具代表性的36份茶树栽培品种的亲缘关系进行分析。从50条引物中筛选出扩增效果良好的16条,共扩增出167条谱带,其中,多态性带为158条(占94.61%),遗传距离的变异范围在0.149~0.679之间。UPGMA聚类结果将36份茶树栽培品种分为3个复合组和2个独立组,聚类结果与茶树品种的来源有关。
【Abstract】 The genetic relationship of 36 tea cultivars grown in Sichuan was studied by RAPD technique.Sixteen primers selected from 50 random primers were used to amplify 36 tea samples,and a total of 167 DNA bands were amplified,among which 158(94.61%)were polymorphic.Genetic distances between the cultivars varied from 0.149 to 0.679.UPGMA cluster analysis of 167 bands amplified by 16 primers was made.The result showed that 36 tea cultivars tested could be classified into 3 complex groups and 2 simple groups.It was found that there was a certain correlation between the results and the derivation of tea cultivars.
【Key words】 Tea plant; Camellia sinensis; RAPD; Genetic relationship; UPGMA;
- 【文献出处】 园艺学报 ,Acta Horticulturae Sinica , 编辑部邮箱 ,2007年01期
- 【分类号】S571.1
- 【被引频次】21
- 【下载频次】192