【摘要】 目的探讨联合检测前S1(Pre-S1)、前S2(Pre-S2)抗原及DNA在乙型肝炎诊断中的意义。方法采用ELISA双抗体夹心法检测乙肝Pre-S2、Pre-S1抗原阳性及同时阳性标本各100例,并以PCR法检测其DNA含量。结果Pre-S1抗原阳性样本100份中,检测出乙肝DNA阳性85份,检出率85%;Pre-S2抗原阳性样本100份中,检测出乙肝DNA阳性83份,检出率83%;Pre-S1、Pre-S2抗原均阳性样本100份中,检测出乙肝DNA阳性93份,检出率93%,明显高于单一抗原检测(P<0.05)。结论乙肝Pre-S1、Pre-S2抗原与其病毒复制有关,两种抗原同时在同一样本中的检出,可作为判断乙肝病毒复制的可靠依据。更多还原

【Abstract】 Objective To study the significance of detecting HBV Per-S1,Per-S2 antigens and their DNA in diagnosing type B hepatitis.Methods ELISA of double antibody sandwich method was used to measure HBV Pre-S1 and Pre-S2,and PCR to detect the DNA content.Results Among 100 examples of only Pre-S1 antigen positive,the positive rate of HBV DNA was 85%.In 100 examples of only Pre-S2 antigen positive,the positive rate of HBV DNA was 83%.In 100 examples of both Pre-S1 and Pre-S2 antigens positive,the positive rate of HBV DNA was 93%,significantly higher than single antigen detection(P <0.05).Conclusion HBV Pre-S1 and Pre-S2 antigens are related to viral replication.HBV replication will be ascertained if the two antigens are detected in the same sample simultaneously.更多还原