【摘要】 利用葡萄糖在镍电极上的电化学氧化．制备了无酶葡萄糖传感器,研究了其电化学氧化机理．并测定了血清中葡萄糖的含量。在较高的pH值和570 mV的电位条件下,镍电极上产生的Ni（Ⅲ）具有氧化剂的作用,能直接氧化葡萄糖为葡萄糖酸内酯,产生的正比于葡萄糖浓度的电流响应可以定量样品中的葡萄糖含量。传感器由镍棒、铂丝对极和Ag/AgCl参比电极构成;对葡萄糖的响应时间小于1 min,进样间隔时间为3 min;对葡萄糖的电流响应范围为1．96×10^-5～1．80×10^-4mol/L,检测限为9．80×10^-6mol/L。传感器未使用葡萄糖氧化酶或其他生物酶,受温度的影响较小,样品中的氧对测定没有影响。在镍电极上,抗坏血酸、尿酸和多巴胺等物质不干扰血清中葡萄糖的测定。传感器用于测定血清中葡萄糖含量,相对标准偏差为4．3％。与己糖激酶法的测定结果一致。传感器制备简单,无需特殊保管,经简单处理后可重复使用。更多还原

【Abstract】 A novel glucose sensor without enzyme was prepared based on the electrochemical oxidation of glucose at a nickel electrode.The mechanism of electrochemical oxidation and response of the sensor to glucose was investigated.In the alkaline solution,the Ni（Ⅲ）species of nickel electrode surface acts as a oxidant for the oxidation of glucose,the glucose is quickly oxidized to glucolactone by the Ni（Ⅲ）species, resulting to the increase of current,which can quantify the concentrations of glucose.The sensor was composed of a nickel rod working electrode,a platinum counter electrode and an Ag/AgCl reference elec- trode.The wave potential of the electrochemical oxidation at 570 mV was employed as the working potential of the sensor.The response time of the sensor is less than 60 seconds and the time of monitoring a sample is 3 minutes.The current response of the sensor is proportional to the concentration of glucose,the detecting range is from 1.96×10^-5 to 1.80×10^-4mol/L,with a detection limit of 9.80×10^-6mol/L.Because the sensor does not contain enzyme,the effect of temperature is less and oxygen in sample does not affect the determination of glucose.Dopamine,ascorbic acid and urea acid do not interfere with the determination of glucose due to their inactivation at nickel electrode.The sensor has been applied to the determination of prac- tical samples,the relative standard deviation（RSD）is less than 4.3%（n=5）for the determination of glucose in human blood serum and the result is consistent with that from the hexokinase method.The preparation of the sensor is simple and has a lower cost,as well as it can be repeatedly used after simple treatment.更多还原