【摘要】 RecA与UvrA是细胞SOS系统中重要的修复蛋白,在细胞DNA损伤后诱导表达.将报告基因绿色荧光蛋白基因(gfpmut3a)分别置于recA和uvrA启动子调控下,构建成质粒pRecAgfp和pUvrAgfp,并转化E.coliDH5α,构建成检测菌株ERS101和EUS101.试验发现,菌株ERS101和EUS101在致畸物吖啶橙处理后,GFP的表达量增高,菌悬液经507nm的蓝光激发后产生的绿色荧光明显增强,且与吖啶橙的浓度具有一定的相关性,即使在0.5μgmL-1的吖啶橙诱导下也能检测到荧光增强效应.对硝基酚等多种致畸物处理后的检测菌株均有荧光增强效应.图4表1参9更多还原

【Abstract】 RecA or UvrA was one of the most important DNA repair proteins in SOS system. Plasmids pRecAgfp and pUvrAgfp were constructed after DNA damage-inducible promoters of recA and uvrA from Escherichia coli were fused to the reporter gene gfpmut3a operon. Genotoxic detectable Strains ERS101 and EUS101 were obtained after introducing plasmids pRecAgfp or pUvrAgfp into E. coli DH5α. Strains ERS101 and EUS101 produced More bioluminescent when treated with genotoxic acridine orange even at 0.5 μg mL-1 concentrations, and the bioluminescent intensity was correlated with the concentration of acridine orange. more bioluminescent was also detected when treated with other genotoxic such as 4-nitrophenol. Fig 4, Tab 1, Ref 9更多还原