【摘要】 目的建立人工蛹虫草子实体多糖的提取与含量测定方法。方法采用正交试验筛选提取条件,苯酚-硫酸法测定多糖的含量。结果多糖的最佳提取条件为80倍量纯化水,45℃提取1次,每次10 min;线性范围为10~100μg.mL-1,r=0.9998,加样回收率为99.41%,RSD=2.36%。3批供试品中多糖的平均含量为22.12%。结论该提取和测定方法操作简单,重复性好,结果准确可靠,可用于人工蛹虫草中多糖的提取与含量测定。更多还原

【Abstract】 Objective To develop a method for extracting and determinating polysaccharides from cultured Cordyceps militaris L.Link.Methods Orthogonal experiment was used to confirm the way of extracting.Contents of polysaccharides were detected by using phenol-sulphate acid.Results The best condition for extracting polysaccharides was by 80 times of pure water,once at 45 ℃,10 min for one time.Linear range was 10～100 g·mL-1,r = 0.999 8,the recovery rate was 99.41%,RSD= 2.36%.The average concentration of polysaccharides in three samples was 22.12%.Conclusion The extracting method is simple,reproducible and the results is accurate and reliable.It can be used to extract and determine the contents of polysaccharides in cultured Cordyceps militaris L.Link.更多还原