【摘要】 目的以壳聚糖为载体材料,多柔比星为模型药物,制备脑内局部给药缓释微球。方法以液体石蜡为油相,L-抗坏血酸棕榈酸酯为交联剂,司盘-80为乳化剂,采用乳化化学交联技术制备多柔比星脑用微球。用动态透析法检测微球的体外释放特性。结果多柔比星/壳聚糖的质量比为1:9的载药微球形态良好,粒径分布较为均匀,平均粒径为(9.41±2.43)μm,载药量为(8.49±0.37)%,包封率为(70.56±4.23)%。体外释放具有良好的缓释效果。结论所优化的制备工艺稳定,适用于多柔比星壳聚糖脑用微球的制备。更多还原

【Abstract】 Objective To study the preparation technique and release characteristic of doxorubicin-loaded chitosan microspheres for the intracerebral local administration. Methods Using the liquid paraffin as the oil phase,L-ascorbyl palmitate as the cross linking agent,and span-80 as the emulsifier.Doxorubicin-loaded chitosan microspheres were prepared by emulsion-chemical cross link technique.Dynamic dialysis method was used to determine the releasing characteristic of microspheres in vitro. Results Microspheres(doxorubicin /chitosan =1:9) with a good shape and even particle size distribution were prepared.The average particle diameter was(9.41±2.43) μm with a quantity of drug loading(8.49±0.37)%.The proportion of entrapment was(70.56±4.23)%.The drug release in vitro had a satisfied effect of delayed release. Conclusion The optimized preparation technique is stable and has a high entrapment efficiency,suitable for the preparation of doxorubicin-loaded chitosan microspheres for cerebral administration.更多还原