【摘要】 目的研究大孔吸附树脂分离纯化五鹤续断总皂苷的工艺,确定最佳工艺条件和参数。方法用95%乙醇溶液提取五鹤续断后,浓缩提取液至无醇味的浸膏,水溶解后上大孔吸附树脂柱,用香草醛-浓硫酸显色反应比色法在波长532 nm测定总皂苷的含量,以总皂苷的含量为考察指标,对影响总皂苷分离纯化的工艺参数进行考察。结果D101型大孔树脂为五鹤续断总皂苷的最佳分离纯化树脂,工艺条件:上样浓度为0.75 mg.mL-1,1 g树脂最大吸附量为0.85 g五鹤续断生药材,洗脱剂为50%乙醇,洗脱剂用量为5倍量树脂柱床体积。结论D101型大孔树脂在所确定的工艺条件下能较好地分离纯化五鹤续断总皂苷,其固形物中总皂苷的含量>50%,总皂苷的回收率>91%。该法适用于工业上大规模制备五鹤续断总皂苷。更多还原

【Abstract】 Objective To study the technology for the separation and purification of total saponin from the "Wu-He" dipsacus root with macroporous adsorption resin as well as to define the optimum technological conditions and parameters. Methods After the "Wu-He" dipsacus root had been extracted with 95% ethanol,the extract was concentrated until no alcoholic smell was left.The extract was then dissolved in water and added onto a macroporous absorption resin column.The content of the total saponin was determined with vanilin-sulfuric acid colorimetry at a detection wavelength of 52 nm.With the use of the concentration of the total saponin as an observation index,the technological parameters that interfered with the separation and purification of the total saponin were investigated. Results The D101 macroporous resin was shown to be the best resin for the separation and purification of the total saponin from the "Wu-He" dipsacus root.The technological conditions were as follows: the concentration of samples added onto the column was 0.75 mg·mL-1,the maximum absorption amount of 1 g of resin was 0.85 g of the crude drug of "Wu-He" dipsacus root,the eluent was 50% ethanol,the optimum flow rate was 1 mL·min-1,5 volumes of the eluent were used for 1 volume of the resin. Conclusion The total saponin in the "Wu-He" dipsacus root was well separated and purified with the D101 macroporous resin under the defined technological conditions.The content of the total saponin was greater than 50% of the solid mateial,and the rate of recovery of the total saponin was higher than 91%.The method is suitable for large-scale industrial preparation of total saponin from "Wu-He" dipsacus root.更多还原