【摘要】 目的牛分枝杆菌与结核分枝杆菌由于99%以上的基因序列相同,从分子生物学角度很难鉴别。本工作从牛分枝杆菌存在pncA基因C169G突变和oxyR基因G285A的不同出发,利用DHPLC和SURVEYOR酶法进行测定,以探讨这两种以异源双链分析为基础的基因突变检测的新方法在这两种分枝杆菌鉴别中的应用。方法DHPLC法和SURVEYOR酶法分析结核分枝杆菌与牛分枝杆菌的pncA及oxyR基因。结果牛分枝杆菌和结核分枝杆菌的DHPLC图谱和SURVEYOR酶法的电泳图谱显著不同,很容易将两者区分开来。结论DHPLC法和SURVEYOR酶法稳定,灵敏,简便,快速,在结核与牛分枝杆菌的鉴别中有一定的作用。更多还原

【Abstract】 Objective It is very difficult to differentiate M.bovis from M.tuberculosis from the aspect of molecular microbiology because of more than 99% similarity of their genome sequence.This work is to differentiate these two mycobacteria by DHPLC and SURVEYOR Nuclease methods according to pncA gene C169G mutation and oxyR gene G285A difference of M.bovis,for exploring the application of these two new methods of gene mutation detection based on heteroduplex analysis.Methods pncA gene and oxyR gene of M.tb and M.bovis were analyzed by DHPLC and SURVEYOR Nuclease methods.Results It was very easy to judge whether a sample was M.tb or M.bovis from DHPLC profile or SURVEYOR electrophoresis bands.Conclusions DHPLC and SURVEYOR Nuclease methods are stable,sensible,simple,rapid and may become new methods to help to differentiate M.bovis from M.tb.更多还原