【摘要】 目的做外周血淋巴细胞中乙肝病毒cccDNA检测研究,并探讨其临床意义。方法采用淋巴细胞分离液分离外周血淋巴细胞,用荧光定量PCR法检测外周血淋巴细胞中cccDNA含量。结果36例CAH患者中检出29例阳性(80.56%),61例CPH患者中检出32例阳性(52.46%),59例ASC标本中检出1例阳性(0.02%)。47例健康人标本检测结果均为阴性。CAH组、CPH组、携带者及阴性对照组之间互相存在统计学差异(P<0.05),携带者组和阴性对照组之间无统计学差异。对定量资料的统计学分析显示:CAH组检测的基因拷贝数较CPH组基因拷贝数明显增高,两者具有统计学差异(P<0.05)。结论(1)cccDNA在外周血淋巴细胞中的存在,对于慢性乙肝病毒持续感染状态的建立和维持,可能具有重要意义。(2)检测外周血淋巴细胞乙肝病毒cccDNA存在及其含量高低,可以作为评估临床抗乙肝病毒治疗有效性的一个参考指标。(3)该检测还可用于乙肝患者治疗中的复发及预后判断。更多还原

【Abstract】 Objective Assaying HBV cccDNA in periphral blood lymphocyte and discuss its clinical significance.Methods Using lymphocyte isolating solution to isolate lymphocytes in the peripheral blood, and quantitative florescent realtime PCR was used to detect HBV cccDNA.Results 29 cases of 36 CAH patients was detected positive(80.56%),32 cases of 61 CPH patients was detected positive(52.46%) and 1 case of 59 ASC was detected positive(0.02%) respectively.All 47 healthy people specimen was found negative by the method.CAH group,CPH group and ASC & negative control group show significance statistically(P<0.05).there is no statistical significance between ASC group and negative control group.Quantitatively,the copies of CAH group are significantly higher than those in CPH group(P<0.05).Conclusions(1)The presence of HBV cccDNA in peripheral blood lymphocyte must have significance in establishment and sustainment of HBV persistency.(2) Assaying cccDNA in PBL can be a useful index to monitor the efficacy of anti-HBV drugs clinically.(3) the assay can also be used to assess relapse and prognosis in clinical scene.更多还原