【摘要】 目的比较人喉鳞癌放射抗拒细胞株Hep-2R与其亲本细胞株Hep-2的体外增殖、运动及侵袭能力。方法分别培养人喉鳞癌放射抗拒细胞株Hep-2R和Hep-2,通过克隆形成实验测定其增殖能力,Transwells小室法测定运动及侵袭能力。结果Hep-2和Hep-2R的克隆形成率分别为(20.75±1.29)%和(19.17±0.82)%,二者有显著性差异(P<0.05);运动力(6个镜下视野的穿膜细胞数)分别为(122.0±6.8)和(117.0±5.8),二者无显著性差异(P>0.05);侵袭力(6个镜下视野的穿膜细胞数)分别为(166.0±9.8)和(166.4±8.4),二者无显著性差异(P>0.05)。结论人喉鳞癌放射抗拒细胞株Hep-2R较其亲本细胞株Hep-2的体外增殖能力弱,而运动和侵袭能力无显著性差异。更多还原

【Abstract】 Objective To compare the abilities of proliferation,mobility and invasion between radioresis-tant human larynx squamous carcinoma cell line Hep-2R and its parent cell line Hep-2 in vitro.Methods The abo-ve-mentioned cell lines were cultured.The ability of proliferation was measured with colony formation.Cell mob-ility and invasion were assayed by transwell chamber model assay.Results The colony formation rates of the two cell lines wer(e20.75±1.29)% and(19.17±0.82)%,P<0.05.Transwell chamber model assay showed the cell mob-ility abilities were(122.0±6.8)and(117.0±5.8),P>0.05;cell invasion abilities were(166.0±9.8)and(166.4±8.4),P >0.05.Conclusion The ability of proliferation in radioresistant human larynx squamous carcinoma cell line Hep-2R was weaker than in its parent cell line Hep-2,but there were no significant differences in mobility and in-vasion abilities between these two cell lines.更多还原